Date of Publication
11-2018
Document Type
Master's Thesis
Degree Name
Master of Science in Biology
Subject Categories
Biology
College
College of Science
Department/Unit
Biology
Thesis Adviser
Ma. Carmen Ablan-Lagman
Defense Panel Chair
Maria Rowena Romana-Eguia
Defense Panel Member
Neil Aldrin C. Mallari
Rachel Ravagoo-Gotanco
Abstract/Summary
The Philippines is a major source of the mangrove crab Scylla serrata. Temperature is an important factor for mangrove crab growth. Three major mangrove crab production sites in the Philippines were chosen for this study: Cagayan, Sorsogon and Oriental Mindoro. Sites were chosen based on its temperature profile and climate type. This study aims to determine differences in thermal stress response of S. serrata populations from sites with different temperature profiles using gene expression and enzyme activity. Differences were reflected in stress response through enzyme markers but not in HSP70 gene expression. Enzyme markers all showed correlation to thermal stress indicating its usefulness as tools for thermal stress evaluation. Mindoro populations were highly stressed compared to Cagayan and Sorsogon populations. Sorsogon populations exhibited stress but showed ability to remediate through increased antioxidant activity. Cagayan populations had the highest tolerance for thermal stress with low HSP70 expression, oxidative stress markers and antioxidant activity. Overall result show there is significant difference among sites and the combined effect of site and temperature. Results complement the East and West genetic population structure based on the populations’ thermal stress response. Eastern populations had higher tolerance to thermal stress than the Western population. Results indicate that S. serrata are ideal species for aquaculture. Matching of population sources to rearing sites must be done to keep thermal-sensitive populations in areas that have similar temperature range to minimize stress and possibly improve crab growth and development.
Abstract Format
html
Language
English
Format
Electronic
Accession Number
CDTG007362
Keywords
Scylla serrata—Effect of temperature on
Upload Full Text
wf_yes
Recommended Citation
Garcia, M. V. (2018). HSP70 expression, oxidative stress and antioxidant capacity response to temperature stress of Scylla serrata from areas with varying temperature profiles. Retrieved from https://animorepository.dlsu.edu.ph/etd_masteral/6309
Embargo Period
9-1-2022
