Date of Publication

4-2020

Document Type

Master's Thesis

Degree Name

Master of Science in Biology

Subject Categories

Biology

College

College of Science

Department/Unit

Biology

Thesis Adviser

Esperanza C. Cabrera

Abstract/Summary

The high prevalence rate of cancer cases worldwide and the adverse side effects of the different cancer treatments encourages researches to find novel plant compounds with promising anti-cancer property. In this study, the biological activities of skullcapflavone I (SKI), a pure compound extracted from the roots of Andrographis paniculata, was evaluated on different cancer cell lines: colorectal (HT-29), breast (MCF-7), leukemia (THP-1), and lung (H69PR); and normal cell line, human neonatal dermal fibroblast (HDFn). PrestoBlue® resazurin viability assays showed that SKI was cytoprotective to HDFn cells against H2O2-induced damage (EC50 = 24.61 µg/mL). PrestoBlue® cell viability assays likewise revealed the cytotoxic activity of SKI against the tested cancer cell lines (IC50 = 3.856 to 9.967 µg/mL), but was significantly not cytotoxic to normal HDFn cells (IC50 > 100 µg/mL). Moreover, SKI exhibited significant genotoxicity in HT-29 cells based on the: tail length (TL) = 41.70 ± 2.65; % tail DNA (D) = 37.95 ± 2.07; tail moment (TM) = 17.93 ± 1.83, of the comets using Comet assay. Furthermore, treatment of HT-29 cells with the IC50 of SKI resulted in the significant up-regulation of the proapoptotic cfos and cjun genes and down-regulation of the key repair genes Akt and Bcl-2, which may explain its experimental cytotoxic and genotoxic actions. From these analyses, it can be inferred that SKI is a promising compound for use as a chemo-preventive agent on normal cells, and as chemotherapeutic agent against different cancer cell lines.

Abstract Format

html

Language

English

Format

Electronic

Physical Description

179 leaves

Keywords

Scutellaria; Cancer cells; Cell lines

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Embargo Period

5-22-2022

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