Date of Publication
10-2-2020
Document Type
Master's Thesis
Degree Name
Master of Science in Chemistry
Subject Categories
Chemistry
College
College of Science
Department/Unit
Chemistry
Thesis Adviser
Drexel H. Camacho
Defense Panel Chair
Marissa G. Noel
Defense Panel Member
Emmanuel V. Garcia
Charito Tranquilan-Aranilla
Abstract/Summary
The production of low molecular weight kappa-carrageenan by irradiation technique has shown to enhance plant growth and stimulate defense mechanism in rice plants. This study embarked on investigating the structural changes in the κ-carrageenan as the irradiation dose increases. Using the optimized dose, the active molecular fragment of irradiated κ-carrageenan was isolated through alcohol precipitation, dialysis, and HPLC techniques. The semi-pure fragments were characterized using UV- Vis, FT-IR, and NMR spectroscopies. Structural changes of κ-carrageenan at absorbed doses 10 and 20 kGy were monitored and the structural fragments of the degraded κ- carrageenan at these doses were proposed to account for the observed spectroscopic data. Ultrasonication of κ-carrageenan was also conducted and optimized to achieve molecular weight suitable for plant growth promotion. ID and 2D NMR analysis afforded structural fragment that accounts for the degradation of κ-carrageenan under ultrasound treatment. Further studies are recommended to synthesize the proposed fragments and investigate its effect on plant growth.
Keywords: Aged irradiated κ-carrageenan, PGP, ultrasonicated κ-carrageenan,
Abstract Format
html
Language
English
Format
Electronic
Physical Description
xv, 182 leaves
Keywords
Irradiation; Ultrasonics
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Recommended Citation
Tecson, M. G. (2020). Isolation and characterization of irradiated and ultrasonicated degradation products of kappa-carrageenan. Retrieved from https://animorepository.dlsu.edu.ph/etd_masteral/5954
Embargo Period
5-4-2022
