Date of Publication
2006
Document Type
Master's Thesis
Degree Name
Master of Science in Chemistry
Subject Categories
Chemistry
College
College of Science
Department/Unit
Chemistry
Thesis Adviser
Consolacion Y. Ragasa
Defense Panel Member
Glenn V. Alea
Alicia M. Aguinaldo
Abstract/Summary
Hibiscus rosa-sinensis afforded 5,7,4-trihydroxyflavanone (Hr-1), and a mixture of hydrocarbons and squalene (Hr14). The structure of Hr-1 was elucidated by extensive 1D and 2D NMR spectroscopy, while the presence of squalene was deduced by comparison of its 1H-NMR spectral data with those reported in the literature. Hr-1 was tested for antimicrobial potential by the agar well method against seven microorganisms. It indicated low antibacterial activity against Staphylococcus aureus, and low antifungal activity against Candida albicans and Trichophyton mentagrophytes. Hr-1 was tested for cytotoxicity using a human lung non-small cell adenocarcinoma (A549) cell line and a representative of other normal cell types, normal chinese hamster ovarian (AA8) cell line. Results of the study indicated that Hr-1, has no linear interpolation with the A549 cell line, thus IC50 could not be computed and Hr-1 is inactive.
Abstract Format
html
Language
English
Format
Electronic
Accession Number
CDTG004348
Shelf Location
Archives, The Learning Commons, 12F Henry Sy Sr. Hall
Physical Description
xiii, 159 p. ; ill. ; 28 cm. + 1 computer optical disc ; 4 3/4 in.
Keywords
Malvaceae; Antimicrobial agents; Anti-infective agents
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Recommended Citation
Rufino, L. A. (2006). Antimicrobial flavonoid from Hibiscus rosa-sinensis Linn. Retrieved from https://animorepository.dlsu.edu.ph/etd_masteral/3561
