Hydrogen sulfide gas degradation by microorganisms isolated from raw sludge
Date of Publication
2000
Document Type
Master's Thesis
Degree Name
Master of Science in Chemical Engineering
Subject Categories
Chemical Engineering
College
Gokongwei College of Engineering
Department/Unit
Chemical Engineering
Thesis Adviser
Marylou M. Uy
Defense Panel Chair
Carlito M. Salazar
Defense Panel Member
Susan Gallardo
Sevillano SB Olano, Jr.
Abstract/Summary
Pseudomonas aeruginosa and Stenotrophomonas maltophilia were both isolated from canal sludge of Metro Manila using thiosulfate medium. The medium used in maintaining and performing the H2S gas uptake experiment was thiosulfate medium with 1g yeast extract. The cell were at its late logarithmic and early stationary phases of growth when gas was injected to the culture. Results showed that Steno.maltophilia exhibited lower capability in degrading H2S gas than Pseu. aeruginosa. Pseu. aeruginosa had totally degraded the gas on the first twenty minutes whereas for Steno. maltophilia, there is a reduction of 73.92 percent. Both microorganisms exhibited sensitivity to low pH. The experiment was conducted under batch condition. Gas volumes of 1,875, 1,259, 937.5 and 625 mL were injected to the Pseu. aeruginosa culture at flow rate of 12.5mL/s. The percentage reduction of H2S gas on the first 120min increased, from 34.61 percent to 73.07 percent, as the gas volume injected is decreased, from 1,250mL to 625ML, respectively, regardless of H2S gas concentration. No change in the number of cells was observed when gas volume injected was less than 1,000mL.
Abstract Format
html
Language
English
Format
Accession Number
TG03167
Shelf Location
Archives, The Learning Commons, 12F Henry Sy Sr. Hall
Physical Description
77 numb. leaves ; 28 cm.
Keywords
Hydrogen sulphide; Gases; Asphyxiating and poisonous; Sewage sludge; Microbial ecology; Bioremediation; Sewage--Purification--Biological treatment
Recommended Citation
Garcia, N. (2000). Hydrogen sulfide gas degradation by microorganisms isolated from raw sludge. Retrieved from https://animorepository.dlsu.edu.ph/etd_masteral/2556
