Isolation, screening, and characterization of Carbaryl-degrading bacteria from rice soil

Date of Publication

1998

Document Type

Master's Thesis

Degree Name

Master of Science in Biology

Subject Categories

Agricultural Economics | Agricultural Science | Agronomy and Crop Sciences | Biotechnology | Microbial Physiology | Organismal Biological Physiology

College

College of Science

Department/Unit

Biology

Thesis Adviser

Dr. Minda Follosco

Defense Panel Chair

Anthony Lee

Defense Panel Member

Clovia Isabel Z. Holdsworth
Dr. Esperanza C. Cabrera

Abstract/Summary

A carbaryl-hydrolyzing bacterium was isolated from flooded rice soil with a history of carbaryl application. The characteristics of the isolate closely resembled those of Pseudomonas aeruginosa. However, it exhibited negative gelatinase reaction and was nonmotile, hence, it was tentatively identified as Pseudomonas sp. The isolate completely degraded carbaryl 32h after incubation based on a micro thin layer chromatography test of carbaryl. The development of brown-colored spots was observed on filter paper strips indicative of a negative reaction to phenols, the hydrolytic product of carbaryl degradation. The occurrence of a brown-colored product on the inoculated minimal medium broths which progressed in color intensity with time further affirmed carbaryl transformation to non-phenolic metabolites. Control set-up showed a consistent positive reaction to carbaryl/phenols throughout the entire 40h incubation period. If the brown colored products proved to be less toxic or non-toxic than the toxic phenols, the isolate could be used to detoxify industrial wastes containing carbaryl and phenols.

Abstract Format

html

Language

English

Format

Print

Accession Number

TG02886

Shelf Location

Archives, The Learning Commons, 12F Henry Sy Sr. Hall

Physical Description

85 leaves

Keywords

Carbaryl; Carbamates; Insecticides -- Physiological effect; Soil microbiology; Soils -- Testing

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