Date of Publication

1996

Document Type

Master's Thesis

Degree Name

Master of Science in Biology

Subject Categories

Biology

College

College of Science

Department/Unit

Biology

Thesis Adviser

Ma. Luisa D. Enriquez

Defense Panel Chair

Florencia G. Claveria

Defense Panel Member

Abelardo Geronimo
Imelda Pagulayan

Abstract/Summary

Pomacea spp. (golden snails) were collected from two sites: pond of Natural Science Research Institute (NSRI), U.P. Diliman and lagoon of De La Salle University-Dasmarinas, Cavite. Samples were injected with colchicine and incubated. Hepatopancreas were stripped out from the specimen collected from the two sites. The tissue was homogenized and cell suspension was prepared. Cells were plated on slides and consequently stained with Giemsa. A total of 100 metaphase cells were screened from each sites. Each spread was analyzed as to chromosome number and morphology (length, centromeric location, and arm ratio).Chromosome number per cell ranged from 8 to 15 from specimen obtained from Cavite while for Diliman, chromosome number ranged from 8 to 14. The highest number of chromosome observed was 12 for the two sites. The karyotype was found to consist of metacentric and submetacentric chromosomes. The average length of chromosomes of collected snails ranged from 4.6 to 5.0 um for U.P. Diliman and 3.6 to 5.3 um for DLSU-Dasmarinas. The measurements were found to be statistically different for the two sites. Arm lengths, arm ratios and centromeric indices were measured and compared.

Abstract Format

html

Language

English

Format

Electronic

Accession Number

TG02542

Shelf Location

Archives, The Learning Commons, 12F Henry Sy Sr. Hall

Physical Description

47 leaves

Keywords

Snails; Gasteropoda; Karyotypes; Chromosomes; Pancreas--Cytology

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