Date of Publication
10-1993
Document Type
Master's Thesis
Degree Name
Master of Science in Biology
Subject Categories
Bacteriology | Biology
College
College of Science
Department/Unit
Biology
Thesis Adviser
Esperanza Cabrera
Defense Panel Chair
Abelardo Geronimo
Defense Panel Member
Adelwisa Ortega
Priscilla Panlasigui
Abstract/Summary
The antibiotic susceptibility patterns of Escherichia coli isolated from rectal swabs of patients from the San Lazaro Hospital were determined using the standardized disk-agar diffusion method. The antibiotics tested were amikacin, chloramphenicol, metilmicin, sulphamethoxazole-trimethoprim and nalidixic acid. Of the isolates tested, 53.44 percent (31 out of 58 isolates) exhibited multiple resistance to chloramphenicol and sulphamethoxazole-trimethoprim, 8.62 percent (5 out of 58 isolates) to either chloramphenicol or sulphamethoxazole-trimethoprim and the remaining 37.94 percent isolates were sensitive to all of the test antibiotics. All of the isolates were sensitive to amikacin, netilmicin and nalidixic acid.Isolates showing resistance to both chloramphenicol and sulphamethoxazole-trimethoprim but sensitivity to nalidixic acid were made to conjugate with E. coli SF 800 and Salmonella C 568 as recipients. All of the 31 isolates tested transferred their resistance to E. coli SF 800. With Salmonella C 568 as recipient however, only 16 out of 31 (51.61 percent) effected transfer of drug resistance. In both conjugation pairs, complete transfer was seen in all the isolates which effected transfer of the drug resistance. The resistance markers from the donor isolates were transferred at a frequency of 3.35 x 10'3 to 3.39 x 10'-1 to the recipient Salmonella C 568.
The multiplicity of drug resistance among the isolates, their conjugative transferability coupled with high frequency of transfer showed the occurrence of plasmid-borne drug resistance in these E. coli test isolates.
Abstract Format
html
Language
English
Format
Accession Number
TG02203
Shelf Location
Archives, The Learning Commons, 12F Henry Sy Sr. Hall
Physical Description
[70] leaves
Keywords
Antibiotics--Testing; Drug resistance in microorganisms; Microorganisms, Effect of drugs on; San Lazaro Hospital (Philippines)
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Recommended Citation
Zabal, J. C. (1993). Conjugative transferability of the antibiotic resistance in fecal isolates of Escherichia coli from the San Lazaro Hospital. Retrieved from https://animorepository.dlsu.edu.ph/etd_masteral/1528
