Plasmid - mediated drug resistance among the intestinal microflora of antibiotic - fed pigs

Date of Publication

1982

Document Type

Bachelor's Thesis

Degree Name

Bachelor of Science in Biology

Subject Categories

Biology

College

College of Science

Department/Unit

Biology

Honor/Award

Awarded as best thesis, 1982

Thesis Adviser

Esperanza Chua

Abstract/Summary

Ampicillin, gentamycin, kanamycin and tetracycline resistant Escherichia coli and Providencia stuartii isolated from pig intestines were tested for in vitro transfer of their resistance plasmids through conjugation with the resident Escherichia coli of the human intestinal tract. All four replications indicated negative plasmid transfer. Nonconjugative plasmids may be due to the lack of RTF or the chromosomal integration of R determinant. Among other factors, ultraviolet irradiation failed to induce plasmid transfer but resulted in the loss of ampicillin resistance. The curing effect of UV, in addition to drug resistance multiplicity, clearly illustrates the plasmid nature of drug resistance in the donor organisms isolated for this study.

Abstract Format

html

Language

English

Format

Print

Accession Number

TU03070

Shelf Location

Archives, The Learning Commons, 12F Henry Sy Sr. Hall

Physical Description

viii, 43 leaves : col. ill. ; 28 cm.

Keywords

Plasmids; Antibiotics

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