Revision and modification of the experiments on Lipids and Enzymes in biochemistry laboratory manual
Date of Publication
1992
Document Type
Bachelor's Thesis
Degree Name
Bachelor of Science in Chemistry
College
College of Science
Department/Unit
Chemistry
Thesis Adviser
Consolacion Ragasa
Defense Panel Member
Wyona Patalinghug
Abstract/Summary
This study is an attempt to evaluate and revise two laboratory experiments in Biochemistry laboratory. Revision of the experiment on lipids is necessary because the solvent used in extraction is highly flammable which causes accidents. The experiment on enzyme inhibition needs to be revised since the type of enzyme inhibition cannot be determined using urea as inhibitor.
A new solvent system was tested for the isolation of total lipids. Results of the study indicated that the solvent system, hexane-isopropanol (3 2), effectively isolated the total lipids as proven by the positive results. The solvent used for the extraction of cholesterol was acetone.
Alanine was tested for possible inhibitory action against -amylase. Results of the study indicated that alanine of different concentration is an effective inhibitor. Glucose as likewise tested but results obtained are not conclusive.
Abstract Format
html
Language
English
Format
Accession Number
TU17515
Shelf Location
Archives, The Learning Commons, 12F, Henry Sy Sr. Hall
Physical Description
46 leaves : illustrations ; 28 cm.
Keywords
Lipids; Enzymes
Recommended Citation
De Mesa, M. C., & Juaneza, A. M. (1992). Revision and modification of the experiments on Lipids and Enzymes in biochemistry laboratory manual. Retrieved from https://animorepository.dlsu.edu.ph/etd_bachelors/8180
