Detection and isolation of soil-transmitted helminth eggs from soil samples collected from selected areas in Pampanga, Philippines
Date of Publication
2018
Document Type
Bachelor's Thesis
Degree Name
Bachelor of Science in Biology
College
College of Science
Department/Unit
Biology
Thesis Adviser
Mary Jane C. Flores
Defense Panel Chair
Mary Jane C. Flores
Abstract/Summary
Diseases caused by soil-transmitted helminths (STH) are considered as neglected tropical diseases that affect over a billion people worldwide mostly from the low and middle income populations. Detection of soil-transmitted helminth eggs in soil is one tool that is used to assess the risk of STH infection. This study isolated, identified, and characterized STH eggs based on morphological characteristics using light microscopy, and then compared the prevalence of STH eggs between the two sites in Pampanga, Philippines. The researchers obtained soil samples (n=200) from two sites for examination using sucrose centrifugation method. The prevalence of STH eggs in the soil samples was recorded at 27% of either Ascaris lumbricoides or Toxocara sp. eggs. Of the 54 positive soil samples, 56% had A. lumbricoides eggs while the other 44% had Toxocara sp. eggs. Of all A. lumbricoides eggs, 30% were identified in their infective stage. There was no significant difference in the prevalence between the two sites. The communities around these sites are at risk of having STH infections due to the contamination of the soil with helminth eggs particularly the infective stage of Ascaris lumbricoides and Toxocara sp.
Abstract Format
html
Language
English
Format
Electronic
Accession Number
CDTU017610
Shelf Location
Archives, The Learning Commons, 12F, Henry Sy Sr. Hall
Physical Description
1 computer disc ; 4 3/4 in.
Keywords
Diseases--Seasonal variations--Philippines; Tropical medicine--Philippines; Rural health--Seasonal variations--Philippines
Recommended Citation
Rasay, D. M., & Tolentino, P. T. (2018). Detection and isolation of soil-transmitted helminth eggs from soil samples collected from selected areas in Pampanga, Philippines. Retrieved from https://animorepository.dlsu.edu.ph/etd_bachelors/6685