Isolation and antibacterial screening of actinomycetes from soil and leaf samples collected within the DLSU campus

Date of Publication

1995

Document Type

Bachelor's Thesis

Degree Name

Bachelor of Science in Biology

College

College of Science

Department/Unit

Biology

Abstract/Summary

A total of forty isolates of actinomycetes were obtained from soil and leaf samples within the DLSU campus. Of theses, 25 came from leaves and 15 came from soil. Isolation used the technique prescribed by Tunac (1993) with Glycerol Yeast Extract Agar (GYEA) as culture medium. Isolates were described in terms of cultural characteristics. Screening for antibacterial properties of the isolates was done using the Cross Streak method. Of the 25 isolates from leaves, 72 percent (18) inhibited Bacillus subtilis, 56 percent (14) inhibited Staphylococcus aureus, and 24 percent (6) inhibited Pseudomonas aeruginosa. Of the 15 isolates from the soil, 47 percent (7) inhibited S. aureus, 40 percent (6) inhibited B. subtilis, and 27 percent (4) inhibited P. aerugenosa. Of the 40 isolates, 60 percent (24) inhibited P. aeruginosa, 53 percent (21) inhibited S. aureus and 25 percent (10) inhibited P. aerugenosa.

Abstract Format

html

Language

English

Format

Print

Accession Number

TU06955

Shelf Location

Archives, The Learning Commons, 12F, Henry Sy Sr. Hall

Physical Description

43 leaves ; Computer print-out.

Keywords

Actinomycetales; Leaves--Microbiology; De La Salle University; Soil micro-organisms; Antibacterial agents; Micro-organisms, Effect of antibiotics on; xx4 Soils--Bacteriology

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