Isolation and antibacterial screening of actinomycetes from soil and leaf samples collected within the DLSU campus
Date of Publication
1995
Document Type
Bachelor's Thesis
Degree Name
Bachelor of Science in Biology
College
College of Science
Department/Unit
Biology
Abstract/Summary
A total of forty isolates of actinomycetes were obtained from soil and leaf samples within the DLSU campus. Of theses, 25 came from leaves and 15 came from soil. Isolation used the technique prescribed by Tunac (1993) with Glycerol Yeast Extract Agar (GYEA) as culture medium. Isolates were described in terms of cultural characteristics. Screening for antibacterial properties of the isolates was done using the Cross Streak method. Of the 25 isolates from leaves, 72 percent (18) inhibited Bacillus subtilis, 56 percent (14) inhibited Staphylococcus aureus, and 24 percent (6) inhibited Pseudomonas aeruginosa. Of the 15 isolates from the soil, 47 percent (7) inhibited S. aureus, 40 percent (6) inhibited B. subtilis, and 27 percent (4) inhibited P. aerugenosa. Of the 40 isolates, 60 percent (24) inhibited P. aeruginosa, 53 percent (21) inhibited S. aureus and 25 percent (10) inhibited P. aerugenosa.
Abstract Format
html
Language
English
Format
Accession Number
TU06955
Shelf Location
Archives, The Learning Commons, 12F, Henry Sy Sr. Hall
Physical Description
43 leaves ; Computer print-out.
Keywords
Actinomycetales; Leaves--Microbiology; De La Salle University; Soil micro-organisms; Antibacterial agents; Micro-organisms, Effect of antibiotics on; xx4 Soils--Bacteriology
Recommended Citation
Villanueva, W. L., & Hernandez, A. C. (1995). Isolation and antibacterial screening of actinomycetes from soil and leaf samples collected within the DLSU campus. Retrieved from https://animorepository.dlsu.edu.ph/etd_bachelors/6144