Morphological appearance, physicochemical characteristics, and in vitro susceptibility to antifungal drugs of Candida albicans biofilm formed on polyvinyl chloride endotracheal and silicone nasogastric tubes
Date of Publication
2019
Document Type
Bachelor's Thesis
Degree Name
Bachelor of Science in Biology
College
College of Science
Department/Unit
Biology
Thesis Adviser
Derick Earl P. Sumalapao
Defense Panel Chair
Mary Jane Flores
Abstract/Summary
The study determined the morphological appearance, physicochemical characteristics, and susceptibility profile of Candida albicans biofilms on polyvinyl chloride endotracheal tube (ETT) and silicone nasogastric tube (NGT). Morphology of C. albicans biofilms revealed morphogenesis of planktonic cells to hyphal forms surrounded by an extracellular matrix over different monitoring times. The planktonic cells were found to be susceptible to fluconazole, miconazole, posaconazole, and voriconazole. Moreover, biofilms formed on both ETT and NGT remained susceptible to fluconazole, posaconazole, and voriconazole but was found to be resistant to miconazole, with significant reduction in the zone of inhibition diameter across monitoring times. In addition, spectral profile assessment via Fourier transform infrared spectroscopy (FTIR) and attenuated transform infrared-FTIR revealed the frequency of specific functional groups associated with the biofilms on both devices. Specific absorbances of O-H, C-H, C=O, C-N, and C-C stretching were revealed by FTIR as prominent peaks observed in C. albicans biofilm on polyvinyl ETT. Silicone NGT exhibited similar results except for the presence of C-N functional group. Similarly, variations of C-H, C=O, and C-C stretching were also identified by ATR-FTIR on both materials. Data obtained from the present study can provide additional information on the biofilm formation of C. albicans on polyvinyl chloride ETT and silicone NGT. The findings of the present study can provide information to further elucidate the resistance mechanism of the organism which can be used in future studies as new insights in the development of new drugs and surface modification of medical devices.
Abstract Format
html
Language
English
Format
Electronic
Accession Number
CDTU017636
Shelf Location
Archives, The Learning Commons, 12F, Henry Sy Sr. Hall
Physical Description
1 computer disc 4/34 inches
Keywords
Azoles; Biofilms; Candida albicans; Fluorescence microscopy; Polyvinyl chloride; Scanning electron microscopy; Silicones
Recommended Citation
Alegre, F. D., & Salazar, P. D. (2019). Morphological appearance, physicochemical characteristics, and in vitro susceptibility to antifungal drugs of Candida albicans biofilm formed on polyvinyl chloride endotracheal and silicone nasogastric tubes. Retrieved from https://animorepository.dlsu.edu.ph/etd_bachelors/6049