The minimum inhibitory concentration of antibiotics against Escherichia coli from urinary tract infection cases of the National Kidney Institute and the conjugative transferability of the resistance in these isolates
Date of Publication
1993
Document Type
Bachelor's Thesis
Degree Name
Bachelor of Science in Biology
College
College of Science
Department/Unit
Biology
Abstract/Summary
Twenty-nine Escherichia coli isolates collected from June 6 to July 17, 1992 from urinary tract infection cases at the National Kidney Institute were tested for their antibiotic resistance patterns using the modified Kirby-Bauer disc-agar diffusion method. The concentrations of the antibiotics used were the prescribed ones for the method, which were based on the peak plasma concentrations of the antibiotics. The minimum inhibitory concentrations (MIC) of the commonly used antibiotics were also determined to find out the resistance of the isolates to the antibiotics based on the peak attainable concentration of the antibiotics in the urine. The percentage of resistant isolates toward the test antibiotics at the plasma level were as follows: ampicillin, 72.41 percent tetracycline, 62.07 percent co-trimoxazole, 48.28 percent chloramphenicol, 44.83 percent tetracycline, 0 percent co-trimoxazole, 48.28 percent chloramphenicol, 37.93 percent and nalidixic acid at 6.90 percent. The conjugative transferability of the multiple antibiotic resistance of fourteen donor E. coli isolates to the recipient (SF-800) was also tested. The results showed complete transfers of the resistance in 28.57 percent of the donors, incomplete transfers in 28.57 percent, and no transfer of resistance in 42.86 percent of the donors.
Abstract Format
html
Language
English
Format
Accession Number
TU06048
Shelf Location
Archives, The Learning Commons, 12F, Henry Sy Sr. Hall
Physical Description
66 leaves ; Computer print-out.
Keywords
Antibiotics; Urinary tract infections; Drug resistance in micro-organisms; Micro-organisms, Effect of drugs on; Philippines, National Kidney Institute
Recommended Citation
Ancheta, J., Capili, W., Garcia, J., & Gutierrez, H. (1993). The minimum inhibitory concentration of antibiotics against Escherichia coli from urinary tract infection cases of the National Kidney Institute and the conjugative transferability of the resistance in these isolates. Retrieved from https://animorepository.dlsu.edu.ph/etd_bachelors/5916
