Fractionation, isolation, and characterization of some Amorphophallus sp, secondary metabolites

Date of Publication

2005

Document Type

Bachelor's Thesis

Degree Name

Bachelor of Science in Biochemistry

College

College of Science

Department/Unit

Chemistry

Thesis Adviser

Anamy Ma. C. Paano

Defense Panel Chair

Wyona Patalinghug

Defense Panel Member

Emmanuel V. Garcia

Marissa G. Noel

Abstract/Summary

The crude alcoholic extracts of stems and leaves as well as tubers and roots were fractionated by sequential extraction. Extracts obtained from this step were set aside for physiological activity testing. The hexane extract of tubers and roots was chosen for further fractionation based on their TLC profile. This extract was fractionated by column chromatography with the aim of getting some pure actions.

The Amorphophallus sp. is a well-known remedial plant for a number of ailments, but reports on the secondary metabolites of Amorphophallus sp. are limited. With the limited reports, there remains a need to bridge the gap between the identified metabolites of these plants and their actual psychological activities. This study partially addressed both concerns.

Thirty-six (36) fractions were obtained from column chromatography. The major non-polar band (Fractions 16 & 17) was obtained as a semi-pure fraction (ACT 1-16). This was not purified further but was analyzed by GC-Ms. Ergosta-5, 22-dien-3-ol, acetate (RT 24.64 min), lupeol (RT 24.65 min), betulin (RT 24.70 min), 3-aceto-12-hydroxyoleanane (RT 24.76 min), and 3-aceto-20-hydroxy-12-taraxastene (RT 34.24 min) were identified from the GC-MS data of this fraction.

A second band composed of fractions 32 to 36 appeared to be with while flaky crystals. The combined fractions (ACT1-36). The white crystals obtained from the recrystallization over methanol. The spectral data analysis of this compound revealed a high molecular weight compound (m/z 1700). The NMR data showed mainly solvent peaks. The sample was dissolved in CDCl3, which did not dissolve the sample. The rest of the fractions were set aside for activity testing.

Abstract Format

html

Language

English

Format

Print

Accession Number

TU17526

Shelf Location

Archives, The Learning Commons, 12F, Henry Sy Sr. Hall

Physical Description

viii, 76, [19] leaves : illustrations (some color) ; 28 cm.

Keywords

Amorphophallus; Amorphous substances; Metabolites

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