Isolation of acid phosphatase from cassava (Manihot Esculenta Crantz syn M. Utilissima Pohl) and kamote (Ipomea Bataas)
Date of Publication
2002
Document Type
Bachelor's Thesis
Degree Name
Bachelor of Science in Chemistry
Subject Categories
Chemistry
College
College of Science
Department/Unit
Chemistry
Defense Panel Member
Lazaro-Llanos, Nancy, Dr., adviser;"Patalinghug, Wyona C., Dr, chair"
Abstract/Summary
Acid Phosphatase was isolated and purified from cassava (Manihot Esculenta Crantz syn M. utilissima Pohl) and Kamote (Ipomea batatas) by ammonium sulfate precipitation, centrifugation, and dialysis. The protein concentration was determined through Biuret's test. The activity of the enzyme was monitored using the conversion of p-nitrophenyl phosphate to p-nitrophenol by acid phosphatase. The colored product of the reaction was determined spectrophotometrically. High Performance Liquid Chromatography was used to confirm the presence of acid phosphatase.
Abstract Format
html
Language
English
Format
Accession Number
TU17489
Shelf Location
Archives, The Learning Commons, 12F, Henry Sy Sr. Hall
Physical Description
x, 91, [2] leaves: illustrations (some color); 28 cm.
Keywords
Cassava; Ipomoea
Recommended Citation
Hernandez, L. A., & Pimenta, L. N. (2002). Isolation of acid phosphatase from cassava (Manihot Esculenta Crantz syn M. Utilissima Pohl) and kamote (Ipomea Bataas). Retrieved from https://animorepository.dlsu.edu.ph/etd_bachelors/5760