Isolation of acid phosphatase from cassava (Manihot Esculenta Crantz syn M. Utilissima Pohl) and kamote (Ipomea Bataas)

Date of Publication

2002

Document Type

Bachelor's Thesis

Degree Name

Bachelor of Science in Chemistry

Subject Categories

Chemistry

College

College of Science

Department/Unit

Chemistry

Defense Panel Member

Lazaro-Llanos, Nancy, Dr., adviser;"Patalinghug, Wyona C., Dr, chair"

Abstract/Summary

Acid Phosphatase was isolated and purified from cassava (Manihot Esculenta Crantz syn M. utilissima Pohl) and Kamote (Ipomea batatas) by ammonium sulfate precipitation, centrifugation, and dialysis. The protein concentration was determined through Biuret's test. The activity of the enzyme was monitored using the conversion of p-nitrophenyl phosphate to p-nitrophenol by acid phosphatase. The colored product of the reaction was determined spectrophotometrically. High Performance Liquid Chromatography was used to confirm the presence of acid phosphatase.

Abstract Format

html

Language

English

Format

Print

Accession Number

TU17489

Shelf Location

Archives, The Learning Commons, 12F, Henry Sy Sr. Hall

Physical Description

x, 91, [2] leaves: illustrations (some color); 28 cm.

Keywords

Cassava; Ipomoea

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