Molecular characterization of selected Colocasia species (Araceae) based on matK and trn H-psbA chlorolast DNA sequences

Date of Publication

2009

Document Type

Bachelor's Thesis

Degree Name

Bachelor of Science in Biology

Subject Categories

Biology

College

College of Science

Department/Unit

Biology

Thesis Adviser

Esperanza Maribel G. Agoo

Abstract/Summary

The chloroplast DNA gene matK, and intergenic spacer trnH-psbA, were PCR amplified from 3 selected Colocasia species (C. affinis Schott., C. gigantea (Blume) Hook.f. and C. esculenta (L.) Schott.). Using DnaSP (Rozas et al., 2003), sequence diversity of matK and trnH-psbA were evaluated. The results revealed that the sequence of trnH-psbA is more diverse in terms of polymorphic sites than matK among C. affinis, C. gigantea and C. esculenta, making it a good DNA barcode. The tree generated using matK sequences, applying Kimura 2 P and UPGMA analyses, support the current infrageneric classification of Colocasia wherein C. affinis and C. esculenta belong to Section Tuberosae, while C. gigantea belongs to Section Caulescentes (Matthews, 1991). This supports the findings of Nguyen et al. (1998) that C. gigantea is most different among and even has closer affinity to Alocasia based on mitochondrial DNA and esterase isozymes.The tree generated using trnH-psbA sequences suggest that C. gigantea and C. esculenta are most related. Neither trnH-psbA nor matK sequences support the biogeographical data provided by the World Checklist of Selected Plant Families (2009).

Abstract Format

html

Language

English

Format

Electronic

Accession Number

CDTU019068

Shelf Location

Archives, The Learning Commons, 12F, Henry Sy Sr. Hall

Physical Description

1 computer disc ; 4 3/4 in.

Keywords

Colocasia; Nucleotide sequence

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4-18-2021

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