Screening for the antimicrobial properties of vegetable extracts on selected test microorganisms
Date of Publication
2011
Document Type
Bachelor's Thesis
Degree Name
Bachelor of Science in Biology
Subject Categories
Biology | Food Microbiology | Plant Biology
College
College of Science
Department/Unit
Biology
Thesis Adviser
Patricio Elvin L. Cantiller
Abstract/Summary
Six different vegetables locally available in the Philippine market were purchased from leading supermarkets in Metro Manila. These included Brassica juncea L. Czern. 1859 (Mustard), Ipomoea aquatica Forssk. 1775 (Water Spinach), Ocimum basilicum L. 1753 (Basil), Cucurbita maxima Duchesne. 1786 (Squash), Sechium edule(Jacq.) Sw. 1800 (Chayote), and Lagenaria siceraria (Molina) Standl. 1930 (Bottle Gourd). The leaf extracts of Water Spinach, Mustard, and Basil and the peel extracts of Squash, Chayote and Bottle Gourd were prepared using the solvent extraction method with Methanol, Ethyl Acetate, and Hexane as extracting solvents. The antimicrobial properties of the vegetable extracts were then screened using the disc agar diffusion method. The following test microorganisms were used: gram-positive bacteria Staphylococcus aureus ATCC 25923 and the gram-negative bacteria Escherichia coli ATCC 35218, and Pseudomonas aeruginosa ATCC 49189. The results showed that all six vegetables exhibited antimicrobial activity on at least one test microorganisms. Mustard, Basil, Squash and Bottle Gourd exhibited inhibition on both E. coli and S. aureus. While Water Spinach inhibited both P. aeruginosa and S. aureus, Chayote only inhibited S. aureus. Methanol extracts showed antimicrobial activities on the most number of test microorganisms.This study further validates the presence of antimicrobial agents in the different parts of the vegetables. This may lead to the discovery of cheaper yet equally effective chemotherapeutic drugs.
Abstract Format
html
Language
English
Format
Electronic
Accession Number
CDTU019104
Shelf Location
Archives, The Learning Commons, 12F, Henry Sy Sr. Hall
Physical Description
1 computer disc ; 4 3/4 in.
Keywords
Foliar diagnosis; Plant extracts--Analysis
Recommended Citation
Legaspi, M. G., & Tiongco, A. Y. (2011). Screening for the antimicrobial properties of vegetable extracts on selected test microorganisms. Retrieved from https://animorepository.dlsu.edu.ph/etd_bachelors/5279
Embargo Period
4-19-2021
