Digitized image enhancement of G-bands in human metaphase chromosome
Date of Publication
1999
Document Type
Bachelor's Thesis
Degree Name
Bachelor of Science in Premed Physics
Subject Categories
Physics
College
College of Science
Department/Unit
Physics
Abstract/Summary
Human chromosomes were obtained from peripheral blood lymphocytes of normal male and female donors using routine microculture technique. Metaphase cells were G-banded by trypsin. Images of good metaphase spreads were captured using a JVC CCD camera magnified at 768 by 493. Chromosome bands were enhanced using the Adobe Photoshop software using the Brightness/Contrast and Levels features. The histogram of each processed image was recorded and the sets of enhanced images were presented to the Research and Biotechnology Division's cytogenetics staff and trainees for evaluation. The results of the evaluation using the brightness/contrast feature showed that 82.22% have values of 20/72 respectively. For enhanced images using various level features, shadows, midtones and highlights, 71.56% have input level values of 80/1.00/240 and output level values of 10/255.
Abstract Format
html
Language
English
Format
Accession Number
TU10414
Shelf Location
Archives, The Learning Commons, 12F, Henry Sy Sr. Hall
Physical Description
120 leaves ; Computer printout
Keywords
Chromosome banding—Imaging, Chromosomes—Imaging
Recommended Citation
Aguspina, M., & Tialegko, M. B. (1999). Digitized image enhancement of G-bands in human metaphase chromosome. Retrieved from https://animorepository.dlsu.edu.ph/etd_bachelors/4262
Embargo Period
2-4-2021
