Conjugative co-transferability of multiple drug resistance and enterotoxigenicity in clinical isolates of Escherichia coli

Date of Publication

1996

Document Type

Bachelor's Thesis

Degree Name

Bachelor of Science in Biology

Subject Categories

Biology

College

College of Science

Department/Unit

Biology

Abstract/Summary

Twelve (12) Escherichia coli isolates from San Lazaro Hospital and Escherichia coli SF800 were analyzed for their antibiograms. Among the drugs tested were nalidixic acid, streptomycin, tetracycline, chloramphenicol, sulfamethoxazole trimethoprim, ampicillin and kanamycin. No isolate was found to have resistance pattern to kanamycin. Escherichia coli isolates from San Lazaro Hospital were used as donors for conjugation because of their multiple drug resistance and sensitivity to nalidixic acid. Escherichia coli SF800 which is resistant to nalidixic acid and sensitive to the other antibiotics was used as recipient.In the Ligated Ileal Loop Assay, no fluid accumulation was observed in the loop containing Escherichia coli SF800, implying that enterotoxin was not produced. The twelve donor isolates produced enterotoxin.In the conjugation experiment, nine (9) out of the 12 donors partially transferred their resistance patterns and all 12 donors transferred their enterotoxigenicity to the recipient Escherichia coli SF800. Nine (9) out of the 12 donor isolates of Escherichia coli were able to co-transfer multiple drug resistance and enterotoxigenicity.

Abstract Format

html

Language

English

Format

Print

Accession Number

TU07337

Shelf Location

Archives, The Learning Commons, 12F, Henry Sy Sr. Hall

Physical Description

43 leaves ;"Computer print-out."

Keywords

Escherichia coli--Microbiology; Drug resistance in microorganisms; Microorganisms—Effect of antibiotics on; Antibiotics—Physiological effect

Embargo Period

1-21-2021

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