Extraction of phenolic compounds from Philippine carabao mango (mangifera indica L.) seed kernel using subcritical water

Date of Publication


Document Type


Degree Name

Bachelor of Science in Chemical Engineering


Gokongwei College of Engineering


Chemical Engineering

Thesis Adviser

Madrazo, Cynthia F., Dr.
Gaspillo, Pag-Asa D., Dr.

Defense Panel Member

Borja, Josephine Q., Dr.
Bacani, Florinda T., Dr.
Dugos, Nathaniel P., Dr., chair/dept. chair
Dungca, Jonathan, Dr., dean


An excessive amount of waste from the consumption of mangoes in the form of peels and seeds are generated. There is a rising need to utilize the waste to maximize its benefits from the mango and to minimize the impact of its waste to the environment.

Utilization of the mango seed kernels for the production of bioactive compounds gives a new purpose. The phenolic compound contents of Philippine carabao mango seed kernels were extracted using subcritical water. The main parameters considered were the external temperature of the subcritical reactor and the solid-to-solvent ratio while keeping the pressure and extraction time constant. The temperature levels were 140 °C, 150 °C, and 160 °C. The solid-to-solvent ratio utilized were 1:20, 1:35, and 1:50 grams of dry weight of the mango seed kernel/mL of solvent (g DW/mL solvent). The experiments comprise 18 runs with each combination of parameters and duplicated.

Based from the results of the experiment, the range of phenolic content obtained was from 24.7 to 79.77 mg GAE/g DW. Both temperature and solid-to-solvent ratio have a significant effect on the yield with a p-value of < 0.001 from the two-way ANOVA analysis. The amount of phenolic compounds increased as temperatures and the amount of solvent were increased. The interaction effects were found to be insignificant with a p-value of 0.0776 indicating the independence of the effects of the parameters. The highest amount of phenolic compounds extracted was 79.77 mg GAE/g DW at 160 °C and 1:50 g DW/mL solvent.

Abstract Format




Accession Number


Shelf Location

Archives, The Learning Commons, 12F Henry Sy Sr. Hall

Physical Description

viii, 46 leaves ; illustrations (some color) ; 29 cm. ; 1 computer disc ; 4 3/4 in.


Mango -- Chemotaxonomy; Plant bioactive compounds; Phenols

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