Extraction of phenolic compounds from Philippine carabao mango (mangifera indica L.) seed kernel using subcritical water
Date of Publication
2015
Document Type
Bachelor's Thesis
Degree Name
Bachelor of Science in Chemical Engineering
Subject Categories
Engineering
College
Gokongwei College of Engineering
Department/Unit
Chemical Engineering
Thesis Adviser
Cynthia F. Madrazo
Pag-Asa D. Gaspillo
Defense Panel Member
Josephine Q. Borja
Florinda T. Bacani
Nathaniel P. Dugos
Jonathan Dungca
Abstract/Summary
An excessive amount of waste from the consumption of mangoes in the form of peels and seeds are generated. There is a rising need to utilize the waste to maximize its benefits from the mango and to minimize the impact of its waste to the environment.
Utilization of the mango seed kernels for the production of bioactive compounds gives a new purpose. The phenolic compound contents of Philippine carabao mango seed kernels were extracted using subcritical water. The main parameters considered were the external temperature of the subcritical reactor and the solid-to-solvent ratio while keeping the pressure and extraction time constant. The temperature levels were 140 °C, 150 °C, and 160 °C. The solid-to-solvent ratio utilized were 1:20, 1:35, and 1:50 grams of dry weight of the mango seed kernel/mL of solvent (g DW/mL solvent). The experiments comprise 18 runs with each combination of parameters and duplicated.
Based from the results of the experiment, the range of phenolic content obtained was from 24.7 to 79.77 mg GAE/g DW. Both temperature and solid-to-solvent ratio have a significant effect on the yield with a p-value of < 0.001 from the two-way ANOVA analysis. The amount of phenolic compounds increased as temperatures and the amount of solvent were increased. The interaction effects were found to be insignificant with a p-value of 0.0776 indicating the independence of the effects of the parameters. The highest amount of phenolic compounds extracted was 79.77 mg GAE/g DW at 160 °C and 1:50 g DW/mL solvent.
Abstract Format
html
Language
English
Format
Accession Number
TU21532
Shelf Location
Archives, The Learning Commons, 12F, Henry Sy Sr. Hall
Physical Description
viii, 46 leaves ; illustrations (some color) ; 29 cm. ; 1 computer disc ; 4 3/4 in.
Keywords
Mango--Chemotaxonomy; Plant bioactive compounds; Phenols
Recommended Citation
Baculo, V. F. (2015). Extraction of phenolic compounds from Philippine carabao mango (mangifera indica L.) seed kernel using subcritical water. Retrieved from https://animorepository.dlsu.edu.ph/etd_bachelors/2891