Isolation and characterization of pink pigmented facultative methylotrophic (PPFM) bacteria from leaves of neem, Azadirachta indica

Rinki Kumar

Date of Publication

2008

Document Type

Bachelor's Thesis

Degree Name

Bachelor of Science in Biology

Subject Categories

Biology

College

College of Science

Department/Unit

Biology

Thesis Adviser

Anthony Lee

Defense Panel Chair

Josefina B. De Jesus

Abstract/Summary

A total of twenty pink pigmented facultative methylotrophic bacteria were isolated from the leaves of neem. All isolates showed pink to orange pink pigmented, round colonies with a smooth glistening surface appearance, entire margin and convex elevation. Most of the colonies were opaque and had a butyrous consistency. Staining showed rod to coccobacilli in shape, Gram negative cells, and containing poly-β-hydroxybutyrate granules. Biochemical analysis showed they were all catalase positive majority of them citrate positive urease positive amylase negative and varied in their oxidase activity. They have the ability to grow on ammonium mineral salt (AMS) agar with methanol, glycerol peptone agar (GPA) and tryptic soy agar (TSA), with some variation in colonial morphology. Based on the observed characteristics, they can be assigned to the genus Methylobacterium. v

Abstract Format

html

Language

English

Format

Electronic

Accession Number

CDTU013937

Shelf Location

Archives, The Learning Commons, 12F, Henry Sy Sr. Hall

Physical Description

1 computer optical disc ; 4 3/4 in.

Keywords

Neem; Neem products

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