Morphological identification, morphometric analysis and comparison of DNA extraction utility of ROSE and TNes-urea methods on six species of Pterocaseio spp (Caesionidae) from Navotas Fish-Port

Date of Publication

2008

Document Type

Bachelor's Thesis

Degree Name

Bachelor of Science in Biology

Subject Categories

Biology

College

College of Science

Department/Unit

Biology

Thesis Adviser

Carmen Lagman

Defense Panel Chair

Josefina B. De Jesus

Abstract/Summary

Morphological identification was successful in classifying six species of Pterocaesio from sixty specimens collected from one mixed species haul in Navotas. Body patterns, specifically longitudinal stripes or blotches and markings on the caudal fins proved most instrumental in the identification. Shape analysis using sixteen morphometric characters derived from ten landmarks was successful in delineating four of the six species. A rotating plot using three PCA axes shows four species: P.tile, P. digramma, P. tesellata and P. randalli, clustered in generalized groups and two species, P. pisang and P. chrysozona, clustered overlapping with each other. The morphometric characters used clustered into three groups: measurements for the head and trunk, measurements from the end of the dorsal fin to the tail area and measurements from the end of the dorsal fin to the base of the caudal peduncles. For DNA extraction, both ROSE and TNES Urea methods were successful and are applicable to the species. ROSE produced DNA from thirteen out of eighteen samples while TNES Urea from fourteen out of eighteen samples.

Abstract Format

html

Language

English

Format

Electronic

Accession Number

CDTU013901

Shelf Location

Archives, The Learning Commons, 12F, Henry Sy Sr. Hall

Physical Description

1 computer optical disc ; 4 3/4 in.

Keywords

Fishes--Morphology; Fishes--Identification

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