Assessment of the protective influence of virgin coconut oil against gentamicin-induced acute renal injury in mice

Date of Publication

1-2017

Document Type

Bachelor's Thesis

Degree Name

Bachelor of Science in Biology

Subject Categories

Biology

College

College of Science

Department/Unit

Biology

Thesis Adviser

Pacifico Eric E. Calderon

Abstract/Summary

This study was a preliminary investigation on the possible nephroprotective effects of VCO against gentamicin-induced acute renal injury in Mus musculus. The study determined whether oral treatment with VCO can reduce the nephrotoxic injury of gentamicin by assessing kidney histopathology. Also, a DPPH analysis of the VCO sample was done to identify the measurement of its antioxidant levels. Thirty (30) female ICR mice were randomly assigned to six treatment groups (n=5), namely: Sham (normal saline solution [NSS]), GEN control (NSS + gentamicin 100mg/kg), VCO 5 (5 mL/kg), VCO 10 (10 mL/kg), and the two groups (VCO 5 mL/kg + gentamicin; and VCO 10 mL/kg + gentamicin). Treatment with either NSS or VCO was done for 21 days via oral gavage. The GEN and experimental groups were given once daily with gentamicin intramuscularly from days 15-21. Twenty four (24) hours following the last dose of gentamicin, kidney excision was done under humanely death. Histopathologic examination of the kidneys was done under light microscopy using a standard numerical grading scale. Histopathologic examination revealed attenuation in the development of the renal tissue injury after treatment with VCO 5 mL/kg but not VCO 10 mL/kg. The results might have been due to alteration of the effects of VCO by the acute renal injury at a higher dose. These findings suggest that oral treatment with VCO for 21 days may not have potential nephroprotective effects in reducing renal tissue injury using this animal model.

Abstract Format

html

Language

English

Format

Electronic

Accession Number

CDTU021785

Shelf Location

Archives, The Learning Commons, 12F, Henry Sy Sr. Hall

Keywords

Coconut oil—Therapeutic use; Acute renal failure—Treatment; Nephrotoxicology

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Embargo Period

5-28-2025

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