Effects of plant growth regulators on callus formation in leaf and node explants of Jatropha pandurifolia Andr.
Date of Publication
1998
Document Type
Bachelor's Thesis
Degree Name
Bachelor of Science in Biology
College
College of Science
Department/Unit
Biology
Abstract/Summary
Node and leaf explants of Jatropha pandurifolia were cultured in Murashige and Skoog medium (MS) supplemented with plant growth regulators benzylaminopurine (BA), a-naphthaleneacetic acid (NAA), and 2,4-dichlorophenoxyacetic acid (2,4-D), alone or in combination. For the node explants, the treatments included MS + BA only at 2.5, 5.0, and 10.0 uM, MS + 10.0 uM BA + NAA or 2,4-D added singly at 1.25 uM, and MS + 10.0 uM BA + NAA and 2,4-D added in combination at 0.1 uM. In leaf explants, MS was supplemented only with NAA and 2,4-D, added alone or in combination at different concentrations. MS without plant growth regulator served as control. All node explants exposed to auxin and cytokinin growth regulators were observed to form calluses while all leaf explants except for the treatment with low concentration of NAA (2.5 uM) also formed calluses. Calluses were produced in leaf and node explants for 10 and 14 days inoculation in plant growth regulators, respectively. The most effective treatment for the production of callus in leaf and node explants were observed in the treatments of MS + 2.5 uM 2,4-D and MS + 10.0 uM BA + 1.25 uM NAA, respectively.
Abstract Format
html
Language
English
Format
Accession Number
TU08418
Shelf Location
Archives, The Learning Commons, 12F, Henry Sy Sr. Hall
Physical Description
23 numb. leaves
Keywords
Growth (Plants); Callus (Botany); Plant propagation--In vitro; Plant tissue culture; Jatropha; Euphorbiaceae; Auxins; Plant hormones
Recommended Citation
Sebastian, O. T. (1998). Effects of plant growth regulators on callus formation in leaf and node explants of Jatropha pandurifolia Andr.. Retrieved from https://animorepository.dlsu.edu.ph/etd_bachelors/1584