Effects of plant growth regulators on callus formation in leaf and node explants of Jatropha pandurifolia Andr.

Date of Publication

1998

Document Type

Bachelor's Thesis

Degree Name

Bachelor of Science in Biology

College

College of Science

Department/Unit

Biology

Abstract/Summary

Node and leaf explants of Jatropha pandurifolia were cultured in Murashige and Skoog medium (MS) supplemented with plant growth regulators benzylaminopurine (BA), a-naphthaleneacetic acid (NAA), and 2,4-dichlorophenoxyacetic acid (2,4-D), alone or in combination. For the node explants, the treatments included MS + BA only at 2.5, 5.0, and 10.0 uM, MS + 10.0 uM BA + NAA or 2,4-D added singly at 1.25 uM, and MS + 10.0 uM BA + NAA and 2,4-D added in combination at 0.1 uM. In leaf explants, MS was supplemented only with NAA and 2,4-D, added alone or in combination at different concentrations. MS without plant growth regulator served as control. All node explants exposed to auxin and cytokinin growth regulators were observed to form calluses while all leaf explants except for the treatment with low concentration of NAA (2.5 uM) also formed calluses. Calluses were produced in leaf and node explants for 10 and 14 days inoculation in plant growth regulators, respectively. The most effective treatment for the production of callus in leaf and node explants were observed in the treatments of MS + 2.5 uM 2,4-D and MS + 10.0 uM BA + 1.25 uM NAA, respectively.

Abstract Format

html

Language

English

Format

Print

Accession Number

TU08418

Shelf Location

Archives, The Learning Commons, 12F, Henry Sy Sr. Hall

Physical Description

23 numb. leaves

Keywords

Growth (Plants); Callus (Botany); Plant propagation--In vitro; Plant tissue culture; Jatropha; Euphorbiaceae; Auxins; Plant hormones

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