Isolation, characterization and screening of hydrocarbon-degrading bacteria in Manila Bay

Date of Publication

1997

Document Type

Bachelor's Thesis

Degree Name

Bachelor of Science in Biology

College

College of Science

Department/Unit

Biology

Abstract/Summary

Two strains of hydrocarbon-degrading bacteria were isolated from Manila Bay using Oil Salts Medium (OSM). They are Gram + and Gram - cocci, which are colored white and yellow, respectively. Both were able to degrade and utilize hydrocarbon in unleaded, leaded and diesel gasoline. The diesel fraction of gasoline was more readily attacked by both strains than the unleaded gasoline. The least to be degraded was the leaded gasoline due to the presence of element Lead (Pb), a heavy metal, that may hinder the hydrocarbon-degrading bacteria from degrading hydrocarbons. The rate of biodegradation based on the use of rotovap were 84.21%, 46.51 % and 35.42% for strain 0001, and 89.47%, 55.81% and 2.78% for strain 0002, respectively.

Abstract Format

html

Language

English

Format

Print

Accession Number

TU07645

Shelf Location

Archives, The Learning Commons, 12F, Henry Sy Sr. Hall

Physical Description

27 leaves

Keywords

Hydrocarbons--Biodegradation; Bacteria--Morphology; Manila Bay; Water--Bacteriology; Water--Analysis

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