Effect of particle size, solvent, and extraction time on tannin extract from spondias purpurea bark through soxhlet extraction
Date of Publication
2014
Document Type
Bachelor's Thesis
Degree Name
Bachelor of Science in Chemical Engineering
Subject Categories
Chemical Engineering
College
Gokongwei College of Engineering
Department/Unit
Chemical Engineering
Thesis Adviser
Florinda T. Bacani
Defense Panel Member
Luis Razon
Anamy Paano
Abstract/Summary
Tannis are phytochemical polyphenols that have wide range of uses in industries such as the leather tanning, textile dyeing, ink, wood adhesive, and wine industries. Their many uses make them significant and in demand in various industries. Throughout time, many different trees have been studied to determine their tannin content. In this study, the tannin content of Spondias purpurea or siniguelas bark was explored due to the lack of information and studies on the species. This study aimed to investigate the effects of particle size, solvent type and extraction time on the extraction of tannin from its bark through Soxhlet extraction. The ratios of solvent used were ethanol-water solutions at 0:1, 1:1, and 0.5:9.5. The extraction time was also varied at 4, 6, and 8 hours. The extracts were then analyzed using a Perkin Elmer High-Performance Liquid Chromatography (HPLC) with UV/VIS detector. The HPLC was performed using a reversed phase C-18 column as the stationary phase, and methanol (Solvent A) and 1:25 aqueous acetic acid solution (Solvent B) as the mobile phase. It was confirmed that extraction parameters varied in this study affected the yield of tannin in the extract. Finer particle sizes can produce a higher yield of tannin as long as agglomeration in the extractor does not occur. Moreover, a longer extraction time produces a higher yield. Solvents with higher amount of ethanol gave better yields of tannin. Pure water, as a solvent, gave the lowest amounts of tannin due to the solubility of proteins the 95% ethanol was the most effective. It was observed that 95% ethanol with the longest extraction time of 8 hours gave the highest percentage yield of 19.19% and 17.13% from 10 grams of bark. The model generated that gave an insignificant lack of fit was quadratic. Using ANOVA, the P-value obtained from the model was less than 0.0001,
Abstract Format
html
Language
English
Format
Accession Number
TU20071
Shelf Location
Archives, The Learning Commons, 12F, Henry Sy Sr. Hall
Physical Description
ix, 89 leaves : illustrations (some colored) ; 28 cm. + ; 1 computer optical disc.
Keywords
Tannin plants; Tanning; Hides and skins
Recommended Citation
Baldosano, H. Y., Castillo, M. G., & Elloran, C. H. (2014). Effect of particle size, solvent, and extraction time on tannin extract from spondias purpurea bark through soxhlet extraction. Retrieved from https://animorepository.dlsu.edu.ph/etd_bachelors/11409