Isolation and purification and characterization of Ciguatera toxins from Thunnus albacares
Date of Publication
2001
Document Type
Bachelor's Thesis
Degree Name
Bachelor of Science in Chemistry
College
College of Science
Department/Unit
Chemistry
Thesis Adviser
Ferdinand F. Fajardo
Maria Christina Z. Floresca
Abstract/Summary
Ciguatera is a disease caused by ciguatoxins, which accumulates in the flesh and viscera of fishes. Ciguatoxins (CTX) are produced by the unicellular, microscopic, benthic dinoflagellate Gambierdiscus toxicus. These ciguatoxins were isolated and purified from yellow fin tuna, Thunnus albacares, acquired from Masinloc, Zambales and Odiongan, Romblon. High Performance Liquid Chromatography (HPLC) and Electrospray ionization Mass Spectroscopy (ESI-MS) were employed to verify the presence of CTX in the fish samples. Mouse bioassay was performed to determine its toxicity. CTX extracted from Romblon samples showed positive results in mouse bioassay and MS analysis.
Abstract Format
html
Language
English
Format
Accession Number
TU17486
Shelf Location
Archives, The Learning Commons, 12F, Henry Sy Sr. Hall
Physical Description
vi, 44 leaves : illustrations ; 29 cm.
Keywords
Yellowfin tuna--Philippines; Poisonous fishes--Toxicology--Philippines; Food contamination--Philippines; Food poisoning--Philippines
Recommended Citation
Nicdao, J. V., & Yturzaeta, K. P. (2001). Isolation and purification and characterization of Ciguatera toxins from Thunnus albacares. Retrieved from https://animorepository.dlsu.edu.ph/etd_bachelors/10107
