Secondary metabolite profile of Yacon black tea extracts
Date of Publication
2010
Document Type
Bachelor's Thesis
Degree Name
Bachelor of Science in Chemistry Minor in Business Studies
College
College of Science
Department/Unit
Chemistry
Thesis Adviser
Anamy Paano
Defense Panel Chair
Nancy Lazaro Llanos
Defense Panel Member
Glenn V. Alea
Emmanuel V. Garcia
Abstract/Summary
Fermentation is a distinguishing step in the preparation of black tea. This step is also known to transform flavonoids to the aflavin and the arubigins whose structures are yet to be confirmed. Since most studies on black tea metabolites used Camella sinensis for tea preparation this study was conducted to partially establish the metabolite profile of yacon (Smallanthus sonchifolius) black tea.
Black tea and the controls were separately prepared and extracted with water. The resulting extracts were concentrated prior to flavonoid extraction. The usual method of flavonoid extraction was followed with one modification. The hydrolyzed water extracts were extracted with DCM before extraction with ethyl acetate and amyl alcohol. Twelve (12) organic extracts were obtained out of the prepared black tea and the controls.
HPLC analysis of each extract showed a very different metabolite profile when compared with the controls. All black tea extracts except the second ethyl acetate extract, showed many components with high retention times. All the controls have one major component of lower retention time (RT 4.2 minutes) with a number of very minor peaks clustered around it (RT 2.62 to 3.71 minutes). The less polar metabolites (higher retention time) noted n black tea extracts are attributed to the transformed metabolites brought about by fermentation.
Abstract Format
html
Language
English
Format
Accession Number
TU17516
Shelf Location
Archives, The Learning Commons, 12F, Henry Sy Sr. Hall
Physical Description
xii, 69 leaves : illustrations (some color) ; 28 cm.
Keywords
Yacon; Smallanthus; Tea; Metabolites
Recommended Citation
Alcabasa, J. J., & Fabico, T. O. (2010). Secondary metabolite profile of Yacon black tea extracts. Retrieved from https://animorepository.dlsu.edu.ph/etd_bachelors/10105