Quantifying and Comparing Biofilm Inhibition and Dispersion Efficacies of Aqueous, Ethanol, and Methanol Trompang elephant (Heliotropium indium) Leaf Extracts Against Escherichia coli

Document Types

Paper Presentation

Research Theme (for Paper Presentation and Poster Presentation submissions only)

Food, Nutrition, and Health (FNH)

School Name

Adamson University

Track or Strand

Science, Technology, Engineering, and Mathematics (STEM)

Research Advisor (Last Name, First Name, Middle Initial)

Gurtiza, John Albert D.

Start Date

25-6-2026 10:30 AM

End Date

25-6-2026 12:00 PM

Zoom Link/ Room Assignment

https://zoom.us/j/91936856247?pwd=oCMfMsh44I2wb0dYsEgoInDJy59bOq.1 Meeting ID: 919 3685 6247 | Passcode: research

Abstract/Executive Summary

Biofilm formation is a critical survival strategy employed by bacteria, enabling them to persist in diverse environments and plays a role in the development of antimicrobial resistance (AMR), partly due to the ability of bacteria, such as Escherichia coli, to form biofilms that reduce antibiotic efficacies. This study investigates the antibiofilm potential of trompang elepante (Heliotropium indicum) leaf extracts, highlighting its potential as a source of inhibitor and dispersal compounds that may contribute to the development of plant-based alternative antimicrobial agents. Specifically, it quantifies and compares the biofilm inhibition and dispersion efficacies of 100% aqueous, ethanolic, and methanolic leaf extracts against E. coli ATCC 25922 and determined whether statistically significant differences exist among extracts compared to a Mueller–Hinton broth (negative control) and a 5% povidone-iodine diluted solution (positive control). Using a true-experimental posttest-only control-group design with purposive sampling. The extracts are prepared through maceration and are evaluated using the crystal violet microtiter plate assay, with optical density measured at 570 nm. Results show that the aqueous extract exhibits the highest mean biofilm inhibition (97.82%) and dispersion (99.52%), compared to the positive control (99.34% and 97.04%, respectively). The methanolic extract shows moderate activity, while the ethanolic extract shows lower activity. One-way ANOVA reveals statistically significant differences among extracts (p< 0.05), and Tukey’s HSD confirms that the aqueous extract is not statistically significantly different from the positive control. These findings indicate that H. indicum, particularly its aqueous extract, possesses strong antibiofilm activity against E. coli, highlighting its potential as a source of inhibitor and dispersal compounds that may contribute to the development of plant-based alternative antimicrobial agents, and supporting the importance of solvent polarity in extracting active phytochemicals.

Keywords

Adamson University; Heliotropium indicum leaf extract; Escherichia coli ATCC 25922; biofilm inhibition; biofilm dispersion; maceration extraction method; aqueous extract; ethanolic extract; methanolic extract

Statement of Originality

yes

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Jun 25th, 10:30 AM Jun 25th, 12:00 PM

Quantifying and Comparing Biofilm Inhibition and Dispersion Efficacies of Aqueous, Ethanol, and Methanol Trompang elephant (Heliotropium indium) Leaf Extracts Against Escherichia coli

Biofilm formation is a critical survival strategy employed by bacteria, enabling them to persist in diverse environments and plays a role in the development of antimicrobial resistance (AMR), partly due to the ability of bacteria, such as Escherichia coli, to form biofilms that reduce antibiotic efficacies. This study investigates the antibiofilm potential of trompang elepante (Heliotropium indicum) leaf extracts, highlighting its potential as a source of inhibitor and dispersal compounds that may contribute to the development of plant-based alternative antimicrobial agents. Specifically, it quantifies and compares the biofilm inhibition and dispersion efficacies of 100% aqueous, ethanolic, and methanolic leaf extracts against E. coli ATCC 25922 and determined whether statistically significant differences exist among extracts compared to a Mueller–Hinton broth (negative control) and a 5% povidone-iodine diluted solution (positive control). Using a true-experimental posttest-only control-group design with purposive sampling. The extracts are prepared through maceration and are evaluated using the crystal violet microtiter plate assay, with optical density measured at 570 nm. Results show that the aqueous extract exhibits the highest mean biofilm inhibition (97.82%) and dispersion (99.52%), compared to the positive control (99.34% and 97.04%, respectively). The methanolic extract shows moderate activity, while the ethanolic extract shows lower activity. One-way ANOVA reveals statistically significant differences among extracts (p< 0.05), and Tukey’s HSD confirms that the aqueous extract is not statistically significantly different from the positive control. These findings indicate that H. indicum, particularly its aqueous extract, possesses strong antibiofilm activity against E. coli, highlighting its potential as a source of inhibitor and dispersal compounds that may contribute to the development of plant-based alternative antimicrobial agents, and supporting the importance of solvent polarity in extracting active phytochemicals.

https://animorepository.dlsu.edu.ph/conf_shsrescon/2026/BoA_FNH/2