Document Types
Paper Presentation
School Code
n/a
School Name
Science and Technology Education Center
Research Advisor (Last Name, First Name, Middle Initial)
Brañanola, Zsarina Grace G.
Abstract/Executive Summary
Hepatic injury is an infection and virus-induced inflammation of the liver that can result in numerous medical conditions. As a response, this study utilizes an abundant resource found in the Philippines, which is Coconut (Cocos nucifera), specifically, the coconuts’ shells which have phenolic compounds that are essential for the suppression of inflammation. These Coconut shells undergo pyrolysis and distillation to produce the Coconut shell liquid smoke (CSLS) which is a digestible aqueous solution. Its efficacy in intervening with hepatic injury was tested through In-vivo experimentation with Sprague Dawley rats. Twelve (n=12) Sprague Dawley rats are injected with 10 mg/kg of Cisplatin to induce hepatic injury and are then are divided into 4 groups with their respective oral treatment: (1) three rats as the baseline group and is given no treatment, (2) three rats as the negative control group and treated with 10 mg/kg Saline solution, (3) three rats as the positive control group and treated with 10 mg/kg Godex DS, and (4) three rats as the experimental group treated with 10 mg/kg CSLS. After monitoring, blood samples from the rats were collected for an SGPT test, which assessed the increase and decrease of ALT (alanine aminotransferase) levels. Using One-Way ANOVA, it resulted with the p-value of 0.02197, thus, the null hypothesis was rejected. Therefore, after understanding the results, and findings, the study was able to draw the conclusion that Coconut Shell Liquid Smoke (CSLS) has an anti-inflammatory activity that can intervene in hepatic injury.
Keywords
Hepatic injury; Coconut Shell Liquid smoke (CSLS); Coconut shell; Anti-inflammatory Activity; Sprague-Dawley Rats
Initial Consent for Publication
yes
Start Date
27-6-2023 3:30 PM
End Date
27-6-2023 5:00 PM
Coconut (Cocos nucifera) Shell Liquid Smoke (CSLS) In-Vivo Anti-Inflammatory Activity for Hepatic-Injury in Sprague Dawley Rats
Hepatic injury is an infection and virus-induced inflammation of the liver that can result in numerous medical conditions. As a response, this study utilizes an abundant resource found in the Philippines, which is Coconut (Cocos nucifera), specifically, the coconuts’ shells which have phenolic compounds that are essential for the suppression of inflammation. These Coconut shells undergo pyrolysis and distillation to produce the Coconut shell liquid smoke (CSLS) which is a digestible aqueous solution. Its efficacy in intervening with hepatic injury was tested through In-vivo experimentation with Sprague Dawley rats. Twelve (n=12) Sprague Dawley rats are injected with 10 mg/kg of Cisplatin to induce hepatic injury and are then are divided into 4 groups with their respective oral treatment: (1) three rats as the baseline group and is given no treatment, (2) three rats as the negative control group and treated with 10 mg/kg Saline solution, (3) three rats as the positive control group and treated with 10 mg/kg Godex DS, and (4) three rats as the experimental group treated with 10 mg/kg CSLS. After monitoring, blood samples from the rats were collected for an SGPT test, which assessed the increase and decrease of ALT (alanine aminotransferase) levels. Using One-Way ANOVA, it resulted with the p-value of 0.02197, thus, the null hypothesis was rejected. Therefore, after understanding the results, and findings, the study was able to draw the conclusion that Coconut Shell Liquid Smoke (CSLS) has an anti-inflammatory activity that can intervene in hepatic injury.
