In vitro antimitotic activity of Orthosiphon aristatus (Cat’s Whiskers) using Allium cepa root assay
Document Types
Paper Presentation
Research Advisor (Last Name, First Name, Middle Initial)
Leah D. Madrazo
Abstract/Executive Summary
Researchers have been investigating other potential treatments for cancer, especially knowing that cancer is a huge health issue in the world. Some of these potential treatments are chemotherapy, radiation therapy, and even medicinal plants. One plant that may have potential anticancer properties as a medicinal plant is Orthosiphon aristatus. So far, the in vitro studies that were conducted on O. aristatus are MTT assay, cell migration assay, caspase inhibition assay, colony formation assay, nuclear condensation by Hoechst 33342 stain, and mitochondrial membrane potential by Rhodamine 123 Stain. There have been limited studies in assessing the antimitotic activity of O. aristatus via Allium cepa root assay. The A. cepa root assay uses the meristematic cells found on the root tips of A. cepa, determining the effects of extracts where these root tips are submerged. It was found that O. aristatus at concentrations of 20 mg/ml, 10 mg/ml, and 1 mg/ml respectively, has significant antimitotic activity with mitotic indices of 1.950 ± 0.365 %, 11.32 ± 0.763 %, and 29.43 ± 3.48 %. This suggests a nonlinear relationship between the concentration of the extracts and mitotic indices. The researchers recommend in vivo and isolation of novel phytochemicals to further explore this determined significant antimitotic activity.
Keywords
Allium cepa root assay; antimitotic activity; medicinal plant; Orthosiphon aristatus; plant extract
Research Theme (for Paper Presentation and Poster Presentation submissions only)
Food, Nutrition, and Health (FNH)
In vitro antimitotic activity of Orthosiphon aristatus (Cat’s Whiskers) using Allium cepa root assay
Researchers have been investigating other potential treatments for cancer, especially knowing that cancer is a huge health issue in the world. Some of these potential treatments are chemotherapy, radiation therapy, and even medicinal plants. One plant that may have potential anticancer properties as a medicinal plant is Orthosiphon aristatus. So far, the in vitro studies that were conducted on O. aristatus are MTT assay, cell migration assay, caspase inhibition assay, colony formation assay, nuclear condensation by Hoechst 33342 stain, and mitochondrial membrane potential by Rhodamine 123 Stain. There have been limited studies in assessing the antimitotic activity of O. aristatus via Allium cepa root assay. The A. cepa root assay uses the meristematic cells found on the root tips of A. cepa, determining the effects of extracts where these root tips are submerged. It was found that O. aristatus at concentrations of 20 mg/ml, 10 mg/ml, and 1 mg/ml respectively, has significant antimitotic activity with mitotic indices of 1.950 ± 0.365 %, 11.32 ± 0.763 %, and 29.43 ± 3.48 %. This suggests a nonlinear relationship between the concentration of the extracts and mitotic indices. The researchers recommend in vivo and isolation of novel phytochemicals to further explore this determined significant antimitotic activity.
