Title

Screening of silver-tolerant bacteria from a major Philippine landfill as potential bioremediation agents

College

College of Science

Department/Unit

Biology

Document Type

Article

Source Title

Ecological Chemistry and Engineering S

Volume

25

Issue

3

First Page

469

Last Page

485

Publication Date

9-1-2018

Abstract

The field of microbial biotechnology has revolutionized the utilization of microorganisms to overcome the problems of environmental pollutions. The present study aimed to identify silver-tolerant isolates and screen their ability to synthesize silver nanoparticles for possible use as bioremediation agents. Seventeen bacterial isolates from soil collected from the Smokey Mountain landfill in Manila, Philippines, were found to tolerate 0.01 M AgNO3 in the culture medium. Molecular and phylogenetic analyses using the 16S rRNA gene sequence identified the isolates as Bacillus cereus, Bacillus subtilis, Bacillus flexus, Bacillus thuringiensis, Alcaligenes faecalis, Achromobacter sp. and Ochrobactrum sp. The formation of silver nanoparticles was evident in the change in color of the reaction mixtures, and was detected through UV-VIS spectroscopy with absorbance peaks at 250-300 nm and 400-450 nm. Scanning electron microscopy revealed the aggregation of diverse shapes of silver nanoparticles with sizes ranging from 70 to 200 nm. The best silver nanoparticle-synthesizing isolates were Alcaligenes faecalis and Bacillus cereus. The results denote the promising microbial technology application of the 17 silver-tolerant isolates in combating the adverse effects of metals and other pollutants in the environment. © 2018 Joan S. Adriano et al., published by Sciendo 2018.

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Digitial Object Identifier (DOI)

10.1515/eces-2018-0032

Disciplines

Biology

Keywords

Bioremediation; Biosynthesis; Sanitary landfills—Microbiology--Philippines; Microbial biotechnology

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