Date of Publication

2-23-2022

Document Type

Bachelor's Thesis

Degree Name

Bachelor of Science in Biology major in Systematics and Ecology

Subject Categories

Biology

College

College of Science

Department/Unit

Biology

Thesis Advisor

Esperanza Maribel G. Agoo

Defense Panel Chair

Chona Camille Vince Cruz-Abeledo

Defense Panel Member

Christian Jordan O. dela Rosa
John Paul Domingo

Abstract/Summary

Family Acanthaceae is widely known for its medicinal and economic values – one of which is genus Andrographis Wall. ex Nees. Andrographis paniculata (Burm.f.) Nees is considered most significant, given its broad range of pharmacological activities. However, the circumscription of genus Andrographis is still unclear, recording different numbers of species across global databases. This study aims to analyze chloroplast gene variability within A. paniculata and between Andrographis spp. to determine a candidate gene barcode for species discrimination, and to conduct phylogenetic tree comparisons based on alignment-based methods. Among rbcL, matK, trnL-F, and trnH-psbA markers, non-coding loci, trnL-F, and psbA-trnH, exhibited higher variability. The candidate marker for genus Andrographis was the trnL-F region. Out of 18 sample Andrographis species, six were successfully discriminated across trnL-F gene trees. The Maximum Likelihood method with bootstrap support performed best, localizing 4 Andrographis spp. into separate clades. However, a single gene locus was insufficient to achieve discriminatory power for the remaining Andrographis spp. Tree topologies were also inaccurate when compared with the reference Andrographis tree and existing taxonomic keys. Utilizing multigene barcodes can further improve species-level discrimination and phylogenetic tree accuracy.

Abstract Format

html

Language

English

Format

Electronic

Physical Description

[vii], 130 leaves

Keywords

Acanthaceae; Phylogeny; Traditional medicine

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Embargo Period

2-22-2022

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