Screening of the antibacterial properties of syzygium polycephaloides (Lipote) against selected clinical isolates

Date of Publication

3-2025

Document Type

Bachelor's Thesis

Degree Name

Bachelor of Science in Biology major in Medical Biology

Subject Categories

Biology

College

College of Science

Department/Unit

Biology

Thesis Advisor

Marigold O. Uba

Defense Panel Chair

Michael B. Ples

Defense Panel Member

Christian Jordan O. Dela Rosa
Patricio Elvin L. Cantiller

Abstract/Summary

Syzygium polycephaloides, or lipote is a native and indigenous plant in the Philippines, traditionally used by the Agta people of the Aurora province and Kalanguya indigenous group of the Ifugao province for its medicinal properties. Despite its known use, there is inadequate research on S. polycephaloides, particularly its antibacterial potential. This study aims to confirm the presence of antibacterial phytochemical components of S. polycephaloides and to screen the antibacterial properties of S. polycephaloides ethanolic leaf extract against selected clinical isolates, Staphylococcus aureus and Klebsiella pneumoniae, and their respective multi-drug- resistant strains, Methicillin-resistant Staphylococcus aureus (MRSA) and ESβL-producing Enterobacterales Klebsiella pneumoniae (ESβL-Kp), using the paper disk-diffusion method and spot test. Phytochemical analysis revealed the leaf extract's presence of sterols, triterpenes, flavonoids, alkaloids, saponins, glycosides, and tannins. Antibacterial activity was tested using the paper disk diffusion and spot test methods with varying extract concentrations of 387.6 mg/5 mL, 193.8 mg/5 mL, 96.88 mg/5 mL, and 48.44 mg/5 mL using dimethyl sulfoxide (DMSO) as a solvent. The findings present that S. polycephaloides ethanolic leaf extract exhibited signs of limited antibacterial activity. K. pneumoniae and MRSA displayed signs of inhibition (ranging from 7.3 ± 2 to 9.43 ± 3 and 6.10 mm to 6.63 mm respectively) but did not reach the expected zone of inhibition of 18mm, as seen by positive control, cefotaxime for K. pneumoniae and at least 27 mm as stated by CLSI guidelines. The researchers suggest testing other parts of the plant, conducting different extraction methods, detecting the number of phytochemical constituents, and performing minimum inhibitory concentration and minimum bactericidal concentration further to investigate the antibacterial properties against clinically significant bacteria.

Abstract Format

html

Language

English

Format

Electronic

Keywords

Endemic plants--Philippines; Medicinal plants--Philippines; Antibacterial agents

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Embargo Period

4-11-2025

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