Date of Publication
4-2025
Document Type
Bachelor's Thesis
Degree Name
Bachelor of Science in Biology major in Medical Biology
Subject Categories
Cancer Biology
College
College of Science
Department/Unit
Biology
Thesis Advisor
Gerard Anthony M. Espiritu
Defense Panel Chair
Eligio Santiago V. Maghirang
Defense Panel Member
Don Ashley O. Malabana
John Martin S. Mondragon
Abstract/Summary
Lung cancer is the leading cause of mortality from cancer in the Philippines, however, it has been difficult to develop chemotherapeutic agents due to developed resistance to existing ones. Angiogenesis is the formation of new blood vessels and is crucial for tumor growth and inhibiting it, termed antiangiogenesis, is a complementary treatment to cancer – now considered as the fourth modality. Carmona retusa is recognized by the Department of Health for its medicinal properties and previous studies where it showed antiangiogenic properties important for tumor suppression. This study was conducted to assess the antiangiogenic effects of C. retusa methanolic leaf extract to H69PR cell lines in ovo Duck Chorioallantoic Membrane Assay. The setups were composed of isotretinoin as positive control (PC) and C. retusa leaf extract in 5 mg/mL (TG5) and 10 mg/mL (TG10) concentrations. Immunohistochemical staining was used to assess tumor invasion, mitosis, necrosis (H&E); and microvascular density (desmin). Analysis of H&E sections showed squamous metaplasia in all treatment groups, and confirmed with chromogranin & synaptophysin stains. Tumor invasion was negative in TG10, while PC was negative for necrosis. Microvascular density (MVD) showed a decrease in average blood vessel formation in all treatment groups when compared to the negative control. To assess statistical significance of MVD, one-way ANOVA was performed and showed significant difference between treatment groups (p-value = 0.03), suggesting inhibition of angiogenesis in the PC, TG10, and TG5 groups. C. retusa leaf extract is as effective as the PC in inhibition of angiogenesis and at a higher concentration, may prevent tumor invasion, however, more investigation is needed.
Abstract Format
html
Language
English
Format
Electronic
Keywords
Blood-vessels—Growth; Cancer invasiveness; Lungs—Cancer; Neovascularization inhibitors Small cell lung cancer
Recommended Citation
Ramil, V. A., Tatlonghari, T. M., Carlos, S. S., & Cadena, G. U. (2025). Angiogenesis and invasion inhibition of Carmona retusa (tsaang gubat) leaf extract in small cell lung cancer (H69PR in ovo culture). Retrieved from https://animorepository.dlsu.edu.ph/etdb_bio/105
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Embargo Period
4-12-2027
