Date of Publication
Master of Science in Biology
College of Science
Esperanza C. Cabrera
The high prevalence rate of cancer cases worldwide and the adverse side effects of the different cancer treatments encourages researches to find novel plant compounds with promising anti-cancer property. In this study, the biological activities of skullcapflavone I (SKI), a pure compound extracted from the roots of Andrographis paniculata, was evaluated on different cancer cell lines: colorectal (HT-29), breast (MCF-7), leukemia (THP-1), and lung (H69PR); and normal cell line, human neonatal dermal fibroblast (HDFn). PrestoBlue® resazurin viability assays showed that SKI was cytoprotective to HDFn cells against H2O2-induced damage (EC50 = 24.61 µg/mL). PrestoBlue® cell viability assays likewise revealed the cytotoxic activity of SKI against the tested cancer cell lines (IC50 = 3.856 to 9.967 µg/mL), but was significantly not cytotoxic to normal HDFn cells (IC50 > 100 µg/mL). Moreover, SKI exhibited significant genotoxicity in HT-29 cells based on the: tail length (TL) = 41.70 ± 2.65; % tail DNA (D) = 37.95 ± 2.07; tail moment (TM) = 17.93 ± 1.83, of the comets using Comet assay. Furthermore, treatment of HT-29 cells with the IC50 of SKI resulted in the significant up-regulation of the proapoptotic cfos and cjun genes and down-regulation of the key repair genes Akt and Bcl-2, which may explain its experimental cytotoxic and genotoxic actions. From these analyses, it can be inferred that SKI is a promising compound for use as a chemo-preventive agent on normal cells, and as chemotherapeutic agent against different cancer cell lines.
Scutellaria; Cancer cells; Cell lines
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Martinez, J. D. (2020). Biological activities of skullcapflavone I from Andrographis paniculata (Burm.f.) nees on immortalized cell lines and its effect on the expression of key repair and pro-apoptotic genes. Retrieved from https://animorepository.dlsu.edu.ph/etd_masteral/5981