Title

Induced spawning of Philippine silver therapon, Leiopotherapon plumbeus (Kner, 1864) using various hormones

Date of Publication

2016

Document Type

Master's Thesis

Degree Name

Master of Science in Biology

College

College of Science

Department/Unit

Biology

Thesis Adviser

Maria Rowena R. Eguia

Defense Panel Chair

Jose Santos Carandang, VI

Defense Panel Member

Frolan A. Aya
Mary Jane C. Flores

Abstract/Summary

The silver perch Leiopotherapon plumbeus, locally known as ayungin, is an endemic freshwater fish that is commercially valuable as it commands a high price in the local market. Local L. plumbeus stocks are observed to be depleting due to excessive fishing and other potential causes such as predation by invasive alien species. Hence, there is a need for an induced breeding protocol to propagate silver therapon and conserve what remains of the fishery resource. In this study, 60 females (total length or TL: 108.5 11.8 mm total body weight or TBW: 20.0 5.9 g) and 120 males (TL: 95.9 10.3 mm TBW: 17.9 4.4 g) were distributed into different treatment groups and injected once intra-muscularly with various hormones. Different dosages of human chorionic gonadotropin (HCG), luteinizing hormone releasing hormone analog (LHRHa) and salmon gonadotropin releasing hormone (sGNRH) were tried and assessed to identify the most effective dosage and hormone that could result to high ovulation, fertilization, and hatching rates. For sGNRH, 20, 30, and 40 µg / kg body weight (BW) were the doses used while 10, 20, and 30 µg/kg BW were used for LHRHa. The dosage used for HCG was 50 IU/g BW.HCG served as the control for this is the hormone that is used in the standard method. Results showed that the use of 20 µg/kg BW sGNRH gave high fertilization and hatching rates. However, for the ovulation rate, 20 µg/kg BW sGNRH showed no significant difference when compared to the other dosages of hormones. The result of this experiment would provide an efficient protocol for the local fishermen so they can produce, on demand, a large supply of this high quality and economically important fish species.

Abstract Format

html

Language

English

Format

Electronic

Accession Number

CDTG006756

Shelf Location

Archives, The Learning Commons, 12F Henry Sy Sr. Hall

Physical Description

1 computer optical disc ; 4 3/4 in.

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