Date of Publication

2012

Document Type

Master's Thesis

Degree Name

Master of Science in Chemistry

Subject Categories

Chemistry

College

College of Science

Department/Unit

Chemistry

Thesis Adviser

Consolacion Y. Ragasa

Defense Panel Chair

Jaime Raul O. Janairo

Defense Panel Member

Glenn V. Alea
Ma. Alicia M. Aguinaldo

Abstract/Summary

The dichloromethane extracts of Euphorbia hirta stems afforded taraxerone (1) from EHS2, a mixture of 25-hydroperoxycycloart-23-en-3-b-ol (2a) and 24-hydroperoxycycloart-25-en-3-b-ol (2b) from EHS4, and another mixture of cycloartenol (3a), lupeol (3b), a-amyrin (3c) and b- amyrin (3d) from EHS1. The leaves afforded a mixture of 3a-3d from EHL1 and a mixture of 2a and 2b from EHL2, while the roots yielded a mixture of 3a-3d from EHR3, a mixture of Lupeol unsaturated fatty acid ester 4a, cycloartenol unsaturated fatty acid ester 4b, α-amyrin unsaturated fatty acid ester 4c, and b-amyrin unsaturated fatty acid ester 4d from EHR4 and a mixture of b-sitosterol 5a and Linoleic acid 5b from EHR5. The structures of Taraxerone, 25-hydroperoxycycloart-23-en-3-b- ol and 24-hydroperoxycycloart-25-en-3-b-ol were elucidated by extensive 1-D and 2-D NMR spectroscopic techniques, while the structures of the other isolates were verified by comparison with published data. EHS2 and EHL2 were subjected to antimicrobial activity tests and both have shown activity against P.aeruginosa (AI=0.2 and 0.3) and S. aureus (AI=0.6 and 0.5). EHL2 also showed activity against C.albicans (AI=0.2) and T. mentagrophyte (AI=0.6). The aforementioned isolates were also subjected to cytotoxicity assay, together with EHS1, and results have shown that only EHL2 has a cytotoxic activity against human colon adenocarcinoma HCT11G (IC50=4.7895) cancer cells and lung cancer cell line AS49 (IC50=3.8992). But compared to Doxorubicin, EHL2 has a less potent cytotoxic activity based on its higher IC50 value.

Abstract Format

html

Language

English

Format

Electronic

Electronic File Format

MS WORD

Accession Number

CDTG005094

Shelf Location

Archives, The Learning Commons, 12F Henry Sy Sr. Hall

Physical Description

243 leaves : ill. ; 1 computer optical disc

Keywords

Dichloromethane; Medicinal plants

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