Bioremediation of chlordane in soil matrix employing land spread technique

Date of Publication


Document Type

Master's Thesis

Degree Name

Master of Science in Chemical Engineering


Gokongwei College of Engineering


Chemical Engineering

Thesis Adviser

Joseph L. Auresenia
Susan M. Gallardo

Defense Panel Chair

Pag-asa D. Gaspillo

Defense Panel Member

Jose Marie U. Lim
Anthony D. Lee


The reduction in the concentration of chlordane in soil matrix employing land spread technique that made use of bioaugmentation and biostimulation as treatment method was utilized in this study. The experiment made use of four bioreactors. Treatments were administered to the two reactors while the other reactor received no treatment whereas one bioreactor was maintained as control. The reactor that received bioaugmentation as treatment was amended with biofilm. The microorganism present in the biofilm was identified as Acinetobacter baumanii and Acinetobacter iwoffi. Soil with no history of pesticide contamination was used in the experiment. The soil was contaminated with 2 ppm chlordane. Parameters such as pH, soil conductivity, dechlorination and reduction in chlordane concentration in the soil were measured. Quantification of microbial population both of the biofilm and indigenous microorganisms in the soil was conducted on the 1st, 15th and 30th day of the experiment. On the 15th day of the experiment, a noted decrease in chlordane concentration was observed. The microbial population in the three (3) reactors where chlordane was administered experienced a transition state wherein most of the colonies did not thrive in chlordane. A decline in microbial population was observed in the three reactors as compared to the control. The decline in the population of the microorganisms can be attributed to the formation of chlordane metabolites. However, on the 30th day of the experiment, those microorganisms that can withstand pressure brought about by the addition of chlordane survived. Results showed 99% drop in the chlordane concentration in thirty (30) days in the bioreactor treated with bioaugmentation and biostimulation.

Abstract Format






Accession Number


Shelf Location

Archives, The Learning Commons, 12F Henry Sy Sr. Hall

Physical Description

vii, 105 leaves ; 28 cm.


Bioremediation; Chlordan; Chlordane; Soils

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