Title

Growth dynamics and biochemical contents of the freshwater chlorophycean microalga DLS001 strain under varied Light-emitting diodes (LEDS) and intensities

Date of Publication

2018

Document Type

Bachelor's Thesis

Degree Name

Bachelor of Science in Biology

Subject Categories

Biology

College

College of Science

Department/Unit

Biology

Thesis Adviser

Dr. Emelina H. Mandia

Defense Panel Chair

Mary Jane C. Flores

Defense Panel Member

Dr. Esperanza Maribel G. Agoo
Mariquit M. De Los Reyes
Cecilia P. Reyes

Abstract/Summary

This study determined the growth response, chlorophyll and protein contents of the green chlorophycean DLS001 strain (cf. Chlorococcum sp.) collected from Carmona River, Binan Laguna, under varied light conditions. The light conditions included intensities of 40 and 80μmol/m²s¹ from white, red, blue, and green light-emitting diodes (LEDs). The growth dynamics were monitored for 30 days via spectrophotometry at 680nm every five days. Subsequent analyses were based on freeze-dried biomass. Cultivates grown under 80μmol/m²s¹ of red light produced the fastest growth with the greatest biomass (250mg/L) and the highest protein content (1.38μg/ml) at 40μmol/m²s¹. The lowest results in these parameters were obtained from blue light. However, the blue light produced the highest chlorophyll a (7.91μg/ml) and b (4.31μg/ml) contents, while the red light yielded the lowest chlorophyll a (2.73μg/ml) and b (1.17μg/ml) contents. These results indicated that light intensity and quality under varied LEDs affect the green chlorophycean DLS001 strain in terms of growth, biomass and protein production in favor of the red light, with the blue light promoting better pigment content.

Abstract Format

html

Language

English

Format

Electronic

Accession Number

CDTU017625

Shelf Location

Archives, The Learning Commons, 12F, Henry Sy Sr. Hall

Physical Description

1 computer disc; 4 3/4 in.

Keywords

Chlorophyll; Biochemistry; Light emitting diodes; Growth factors

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