Title

Isolation and crystallization of urease from Garbanzos (Cicer Arietinum), Black Sitaw (Vigna Sinensis), and Green Mongo (Phaseolus Aureus) beans

Date of Publication

2001

Document Type

Bachelor's Thesis

Degree Name

Bachelor of Science in Chemistry

Subject Categories

Chemistry

College

College of Science

Department/Unit

Chemistry

Defense Panel Member

Lazaro-Llanos, Nancy, Dr., adviser;"Patalinghug, Wyona C., Dr, chair"

Abstract/Summary

Urease was isolated using an acetone-water extraction from three local beans namely garbanzos, black sitaw, and green mongo and was crystallized. There were a total of four trials per sample. The protein concentration of each sample was determined using Biuret's test. The activity of the enzyme was monitored using the reaction of urease on urea. The colored product of the reaction was determined spectrophotometrically. High Performance Liquid Chromatography was used to confirm the presence of urease. The amount and activity of urease extracted from the three local beans was compared.

Abstract Format

html

Language

English

Format

Print

Accession Number

TU17490

Shelf Location

Archives, The Learning Commons, 12F, Henry Sy Sr. Hall

Physical Description

xii, 75 leaves: illustrations (some colored); 28 cm.

Keywords

Chickpea; Beans; Cowpea; Mung bean

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