Title

Isolation, purification, and characterization of DNA from capsicum species (Capsicum annuum var longum) found in the Philippines

Date of Publication

2004

Document Type

Bachelor's Thesis

Degree Name

Bachelor of Science in Chemistry

Subject Categories

Chemistry

College

College of Science

Department/Unit

Chemistry

Thesis Adviser

Nancy Llanos Lazaro

Defense Panel Chair

Wyona Patalinghug

Defense Panel Member

Julita Robles
Marissa Noel

Abstract/Summary

There are various medical purposes that had been found to be possessed by Capsicum species. In this study, the DNA from Siling Haba or Louisiana Long Pepper (Capsicum annuum var longum) found in the Philippines was isolated. The protocol utilizes fresh leaves sample from Pedefco Social Farm located at Malaining Bago Naic, Cavite, Philippines. These leaves were grounded with mortar and pestle, several plant cell contaminants such as polysaccharide and polyphenolic compounds were present which prevents the proper isolation and purification of the plant DNA. These contaminants were reduced with the presence of extraction buffer, PVP, and mercaptoethanol. DNA samples were then divided into two groups one containing RNase and the other without. Characterization of the DNA were made by introducing restriction enzymes such as EcoRI, HindIII and the mixture of both (EcoRI/HindII). These fragments were separated using agarose gel electrophoresis and viewed using ethidium bromide stain with the aid of a UV-transilluminator and were properly documented using a digital camera.

Abstract Format

html

Language

English

Format

Print

Accession Number

TU17494

Shelf Location

Archives, The Learning Commons, 12F, Henry Sy Sr. Hall

Physical Description

viii, 53 leaves ; illustrations (some color) ; 28 cm.

Keywords

Peppers--Philippines

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