Determination of tetracyclines in chicken meat samples by solid phase extraction and high performance liquid chromatography

Date of Publication


Document Type

Bachelor's Thesis

Degree Name

Bachelor of Science in Chemistry Minor in Business Studies

Subject Categories



College of Science



Thesis Adviser

Lourder Guidote

Defense Panel Chair

Joan Candice V. Ondevilla

Defense Panel Member

Peter Immanuel Tenido


Antibiotics are dangerous threats if taken in regularly, even in trace amounts. It acts as a trigger for antibiotic resistance in bacteria, which renders common antibiotics useless. In this study, two tetracycline drugs, namely doxycycline and oxytetracycline were analyzed in locally sourced chickens from Bulacan, Philippines for antibiotic residues. HPLC analysis was done to determine the concentrations of the antibiotics. Standard calibration curves from concentration of 100 ppm, 80 ppm, 40 ppm, 10 ppm, 1 ppm, 10 ppb and 10 ppb of the tetracyclines produced a linearity of 0.995 and 0.9994 for doxycycline and oxytetracycline, respectively. 19 chicken samples were tested: 2 spiked samples, 2 blank samples and 15 unknown samples. The spiked samples were able to confirm the antibiotic peak in the samples. However, the blank samples of doxycycline emitted the same chromatogram as the unknown samples which led to two variations in the computation for the concentration, uncorrected and corrected. The known samples had an average retention times of 13.9845 minutes for doxycycylines and 7.090 minutes for oxytetracyline. Average uncorrected concentration for doxycycline is 1.1722 ppm and the average corrected concentration is 0.8384 ppm with a difference of 28%. Oxytetracycline was not found in the meat samples. LOD and LOQ is 0.2651 ppm and 0.8033 ppm for doxycycline, respectively and 2.821 ppm and 8.854 for oxytetracycline. Doxycycline concentrations were found to be above the regulation standards set by the EU.

Abstract Format






Accession Number


Shelf Location

Archives, The Learning Commons, 12F, Henry Sy Sr. Hall

Physical Description

viii, 47, [50] leaves, illustrations (some color), 28 cm.


Antibiotics in animal nutrition--Philippines--Bulacan; Antibiotics--Analysis

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