Inhibition of p66Shc by Camellia sinensis Flavanols: A Potential Strategy Against Oxidative Stress in Alzheimer’s Disease

Proponent/s Name/s (Last Name, First Name, Middle Initial)

Simone Bernice S. Concepcion, De La Salle University, ManilaFollow
Rex Amadeus D. Fanged, De La Salle University, ManilaFollow
Amanda Skye C. Payumo, De La Salle University, ManilaFollow
Lanz Jakob E. Raboy, De La Salle University, ManilaFollow
Christian Jordan O. Dela Rosa, De La Salle University, ManilaFollow
Rosita R. Roldan-Gan, De La Salle University, ManilaFollow
Jane Abigail M. Santiago-Santos, De La Salle University, ManilaFollow

Document Types

Paper Presentation

School Name

De La Salle University

Track or Strand

Science, Technology, Engineering, and Mathematics (STEM)

Research Advisor (Last Name, First Name, Middle Initial)

Santiago-Santos, Jane Abigail, M.

Start Date

23-6-2025 1:30 PM

End Date

23-6-2025 3:00 PM

Zoom Link/ Room Assignment

Y306

Abstract/Executive Summary

Reactive oxygen species (ROS) play essential roles in cellular processes, including immune defense and cell signaling. However, overproduction of the p66Shc protein leads to increased ROS levels and oxidative stress, greatly contributing to the progression of Alzheimer’s Disease (AD). This study investigates the inhibitory potential effects of flavanols from the Camellia sinensis plant on p66Shc to reduce ROS generation. These flavanols examined include catechin, epicatechin gallate (ECG), epigallocatechin gallate (EGCG), gallocatechin gallate (GCG), epigallocatechin (EGC), epicatechin (EC), gallocatechin (GC). A homology model of p66Shc was constructed, and molecular docking simulations were performed to explore the flavanols and p66Shc interactions. One-way ANOVA testing showed significant differences in binding affinities, with GCG showing the strongest interaction (-7.6 kcal/mol) via Van Der Waals interactions. ECG and EGCG followed (-6.9 kcal/mol) forming additional Pi-Anion and Pi-Alkyl interactions. Catechin had a binding score of -6.4 kcal/mol, while the remaining flavanols had a score of -6.2 kcal/mol with varied interactions. These findings suggest that GCG, ECG, and EGCG could be explored further for their potential role in mitigating oxidative stress in AD.

Keywords

p66Shc; green tea flavanols; molecular docking; reactive oxygen species (ROS); Camellia sinensis

Research Theme (for Paper Presentation and Poster Presentation submissions only)

Food, Nutrition, and Health (FNH)

Initial Consent for Publication

no

Statement of Originality

yes