The Effect of Tropical Temperature Conditions on Salmonella Typhimurium Growth
Document Types
Paper Presentation
School Name
Adamson University
Track or Strand
Science, Technology, Engineering, and Mathematics (STEM)
Research Advisor (Last Name, First Name, Middle Initial)
Gurtiza, John Albert D.
Start Date
25-6-2025 10:30 AM
End Date
25-6-2025 12:00 PM
Zoom Link/ Room Assignment
https://zoom.us/j/91578705138?pwd=i2W0YnQc0boXaoi6aX2XBG0uLpFMez.1 Meeting ID: 915 7870 5138 Passcode: FNH3
Abstract/Executive Summary
Salmonella contamination remains a significant health concern, particularly in environments where temperature fluctuations influence bacterial survival. While previous studies have identified temperature as a key factor in microbial growth, the specific effects of varying heat levels on Salmonella enterica serovar Typhimurium concentration require further investigation, particularly in the context of the Philippines. This study examined bacterial concentration across different temperatures by assessing S. typhimurium under controlled laboratory conditions at 37 °C, 42 °C, and 47 °C. Microbiological analysis quantified bacterial colonies (CFU/mL), revealing a decreasing trend in S. typhimurium concentration as temperature increased. The highest concentration was observed at 37 °C, while the lowest occurred at 47 °C, consistent with studies on other serovars indicating that higher temperatures inhibit growth. Statistical analysis found no significant difference among the three temperatures (p = 0.13), suggesting that while temperature affects S. typhimurium growth, additional environmental factors may also play a role. Further research should explore a broader temperature range, including values closer to S. typhimurium’s minimum growth threshold, and examine the effects of prolonged exposure, humidity, and pH. A deeper understanding of S. typhimurium’s thermal adaptation is essential for refining food safety policies and contamination control strategies.
Keywords
Salmonella typhimurium; temperature; bacterial concentration; microbiological analysis; food safety
Research Theme (for Paper Presentation and Poster Presentation submissions only)
Food, Nutrition, and Health (FNH)
Initial Consent for Publication
yes
Statement of Originality
yes
The Effect of Tropical Temperature Conditions on Salmonella Typhimurium Growth
Salmonella contamination remains a significant health concern, particularly in environments where temperature fluctuations influence bacterial survival. While previous studies have identified temperature as a key factor in microbial growth, the specific effects of varying heat levels on Salmonella enterica serovar Typhimurium concentration require further investigation, particularly in the context of the Philippines. This study examined bacterial concentration across different temperatures by assessing S. typhimurium under controlled laboratory conditions at 37 °C, 42 °C, and 47 °C. Microbiological analysis quantified bacterial colonies (CFU/mL), revealing a decreasing trend in S. typhimurium concentration as temperature increased. The highest concentration was observed at 37 °C, while the lowest occurred at 47 °C, consistent with studies on other serovars indicating that higher temperatures inhibit growth. Statistical analysis found no significant difference among the three temperatures (p = 0.13), suggesting that while temperature affects S. typhimurium growth, additional environmental factors may also play a role. Further research should explore a broader temperature range, including values closer to S. typhimurium’s minimum growth threshold, and examine the effects of prolonged exposure, humidity, and pH. A deeper understanding of S. typhimurium’s thermal adaptation is essential for refining food safety policies and contamination control strategies.
https://animorepository.dlsu.edu.ph/conf_shsrescon/2025/paper_fnh/7
