Cytotoxicity of Glucosinolates Extracted from Green Cabbage (Brassica oleracea var. capitata)

Document Types

Paper Presentation

School Name

Caloocan City Science High School

Track or Strand

Science, Technology, Engineering, and Mathematics (STEM)

Research Advisor (Last Name, First Name, Middle Initial)

Dizon, Suzzeth U.

Start Date

25-6-2025 10:30 AM

End Date

25-6-2025 12:00 PM

Zoom Link/ Room Assignment

https://zoom.us/j/91578705138?pwd=i2W0YnQc0boXaoi6aX2XBG0uLpFMez.1 Meeting ID: 915 7870 5138 Passcode: FNH3

Abstract/Executive Summary

Cancer is a large group of diseases characterized by the uncontrolled growth of abnormal cells, which can invade any organ or tissue. According to WHO (2025), cancer is the leading cause of death worldwide, accounting for nearly 10 million deaths in 2020, or approximately one in six deaths. Despite significant advancements in cancer treatments over the years, such as radiation and chemotherapy, commonly used post-surgical complementary therapies can cause adverse side effects, including nerve damage, fatigue, and chronic pain. Glucosinolate (GSLs) is a naturally occurring compound found in cruciferous vegetables, such as green cabbage. GSLs produce metabolites exhibiting anticancer properties (Connolly et al., 2021); however, their cytotoxicity and effect on human cells require further investigation. This study investigates the cytotoxicity of glucosinolates extracted from green cabbage (Brassica oleracea var. capitata). The cytotoxicity of the treatments, Pure Glucosinolate Extract (PGE), Glucosinolate with Water (GEW), and Glucosinolate Hydrolysate (GCH), was evaluated using the Brine Shrimp Lethality Assay (BSLA). The dose-response relationship and the lethal concentration 50 (LC50) toxicity level were determined using probit analysis across various concentrations (5, 10, 100, and 1000 μg/mL) of the glucosinolate-based treatments. The results demonstrated a concentration-dependent effect, with higher doses increasing mortality rates. Among the treatments, PGE exhibited the highest toxicity, followed by GEW, which demonstrated moderate toxicity, while GCH was classified as non-toxic. In particular, the Pure Glucosinolate Extract showed the highest cytotoxicity.

Keywords

anticancer, brine shrimp, cytotoxicity, glucosinolate, green cabbage

Research Theme (for Paper Presentation and Poster Presentation submissions only)

Food, Nutrition, and Health (FNH)

Statement of Originality

yes

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Jun 25th, 10:30 AM Jun 25th, 12:00 PM

Cytotoxicity of Glucosinolates Extracted from Green Cabbage (Brassica oleracea var. capitata)

Cancer is a large group of diseases characterized by the uncontrolled growth of abnormal cells, which can invade any organ or tissue. According to WHO (2025), cancer is the leading cause of death worldwide, accounting for nearly 10 million deaths in 2020, or approximately one in six deaths. Despite significant advancements in cancer treatments over the years, such as radiation and chemotherapy, commonly used post-surgical complementary therapies can cause adverse side effects, including nerve damage, fatigue, and chronic pain. Glucosinolate (GSLs) is a naturally occurring compound found in cruciferous vegetables, such as green cabbage. GSLs produce metabolites exhibiting anticancer properties (Connolly et al., 2021); however, their cytotoxicity and effect on human cells require further investigation. This study investigates the cytotoxicity of glucosinolates extracted from green cabbage (Brassica oleracea var. capitata). The cytotoxicity of the treatments, Pure Glucosinolate Extract (PGE), Glucosinolate with Water (GEW), and Glucosinolate Hydrolysate (GCH), was evaluated using the Brine Shrimp Lethality Assay (BSLA). The dose-response relationship and the lethal concentration 50 (LC50) toxicity level were determined using probit analysis across various concentrations (5, 10, 100, and 1000 μg/mL) of the glucosinolate-based treatments. The results demonstrated a concentration-dependent effect, with higher doses increasing mortality rates. Among the treatments, PGE exhibited the highest toxicity, followed by GEW, which demonstrated moderate toxicity, while GCH was classified as non-toxic. In particular, the Pure Glucosinolate Extract showed the highest cytotoxicity.

https://animorepository.dlsu.edu.ph/conf_shsrescon/2025/paper_fnh/4