Cytotoxicity of Ageratina riparia (Mistflower) Biosynthesized Silver Nanoparticles in Allium cepa Assay

Document Types

Paper Presentation

School Name

University of Baguio Science High School

Track or Strand

Science, Technology, Engineering, and Mathematics (STEM)

Research Advisor (Last Name, First Name, Middle Initial)

Palcongan, Abegail B.

Start Date

25-6-2025 1:00 PM

End Date

25-6-2025 2:30 PM

Zoom Link/ Room Assignment

https://zoom.us/j/97662707236?pwd=0HjilHa9kcSGcxykP1SqHPcIfECfSv.1 Meeting ID: 976 6270 7236 Passcode: 441153

Abstract/Executive Summary

The use of green-synthesized nanoparticles offers a promising route toward eco-friendly and biocompatible alternatives in biomedical research. This study aimed to assess the cytotoxicity of silver nanoparticles (AgNPs) biosynthesized from Ageratina riparia extract. Given the growing interest in plant-mediated nanoparticle synthesis for safe therapeutic applications, evaluating their biological effects is crucial. The extract was obtained through maceration, and green synthesis was conducted using silver nitrate solutions (1 mM, 3 mM, 5 mM). The mixtures were boiled to isolate nanoparticle pellets, which were then redispersed in distilled water for use as treatment liquids in the Allium cepa assay. Results showed that all concentrations of A. riparia-mediated AgNPs were non-cytotoxic, exhibiting no dose-dependent toxicity, while the positive control (0.7% H₂O₂) caused complete chromosomal aberrations. The findings suggest that biosynthesized AgNPs are stable and biocompatible, contributing new evidence to the safety profile of green-synthesized nanoparticles. These results support the potential practical use of A. riparia-based AgNPs in applications requiring low toxicity.

Keywords

Ageratina riparia, biosynthesis, silver nanoparticles, cytotoxicity, Allium cepa assay

Research Theme (for Paper Presentation and Poster Presentation submissions only)

Food, Nutrition, and Health (FNH)

Statement of Originality

yes

This document is currently not available here.

Share

COinS
 
Jun 25th, 1:00 PM Jun 25th, 2:30 PM

Cytotoxicity of Ageratina riparia (Mistflower) Biosynthesized Silver Nanoparticles in Allium cepa Assay

The use of green-synthesized nanoparticles offers a promising route toward eco-friendly and biocompatible alternatives in biomedical research. This study aimed to assess the cytotoxicity of silver nanoparticles (AgNPs) biosynthesized from Ageratina riparia extract. Given the growing interest in plant-mediated nanoparticle synthesis for safe therapeutic applications, evaluating their biological effects is crucial. The extract was obtained through maceration, and green synthesis was conducted using silver nitrate solutions (1 mM, 3 mM, 5 mM). The mixtures were boiled to isolate nanoparticle pellets, which were then redispersed in distilled water for use as treatment liquids in the Allium cepa assay. Results showed that all concentrations of A. riparia-mediated AgNPs were non-cytotoxic, exhibiting no dose-dependent toxicity, while the positive control (0.7% H₂O₂) caused complete chromosomal aberrations. The findings suggest that biosynthesized AgNPs are stable and biocompatible, contributing new evidence to the safety profile of green-synthesized nanoparticles. These results support the potential practical use of A. riparia-based AgNPs in applications requiring low toxicity.

https://animorepository.dlsu.edu.ph/conf_shsrescon/2025/paper_fnh/19