A Comparative Analysis of Maceration Extraction Methods and Their Efficacy in Biofilm Inhibition by Annona muricata Leaf Extract Against Bacillus subtilis
Document Types
Paper Presentation
School Name
Adamson University
Track or Strand
Science, Technology, Engineering, and Mathematics (STEM)
Research Advisor (Last Name, First Name, Middle Initial)
Gurtiza, John Albert D.
Start Date
23-6-2025 1:30 PM
End Date
23-6-2025 3:00 PM
Zoom Link/ Room Assignment
Y306
Abstract/Executive Summary
Biofilms, ubiquitous microbial communities encased in a self-produced matrix, pose a significant threat to human health, industrial processes, and food safety. While synthetic antimicrobials are widely used, concerns about resistance and environmental impact drive the search for natural alternatives. Guyabano (Annona muricata), a tropical fruit known for its antimicrobial properties, shows potential as a natural antibiofilm agent. This study aimed to compare the impact of three different maceration extraction methods (aqueous, ethanolic, and methanolic) on the antibiofilm efficacy of A. muricata leaf extract against Bacillus subtilis biofilm. Each extract was subjected to a 96-well microplate biofilm inhibition assay, measuring optical density (OD) at 600 nm to quantify biofilm formation. Serial dilution and colony-forming unit (CFU) counting were used to assess bacterial viability. The study found that all three extracts demonstrated notable biofilm inhibition, with the highest inhibition observed at a 10−7dilution for ethanolic extract (89.56%). However, the extracts exhibited inconsistent antibacterial activity, with bacterial colonies too many to count (TMTC) at higher dilutions. Statistical analysis revealed no significant difference in the efficacy among the maceration extraction methods (p = 0.67), confirming that all are equally viable for obtaining bioactive compounds from A. muricata. Regardless of the method used, the extracts effectively inhibited B. subtilis biofilm formation.
Keywords
Annona muricata leaf extract; Bacillus subtilis biofilm formation; aqueous extraction; ethanolic extraction; methanolic extraction
Research Theme (for Paper Presentation and Poster Presentation submissions only)
Food, Nutrition, and Health (FNH)
Initial Consent for Publication
yes
Statement of Originality
yes
A Comparative Analysis of Maceration Extraction Methods and Their Efficacy in Biofilm Inhibition by Annona muricata Leaf Extract Against Bacillus subtilis
Biofilms, ubiquitous microbial communities encased in a self-produced matrix, pose a significant threat to human health, industrial processes, and food safety. While synthetic antimicrobials are widely used, concerns about resistance and environmental impact drive the search for natural alternatives. Guyabano (Annona muricata), a tropical fruit known for its antimicrobial properties, shows potential as a natural antibiofilm agent. This study aimed to compare the impact of three different maceration extraction methods (aqueous, ethanolic, and methanolic) on the antibiofilm efficacy of A. muricata leaf extract against Bacillus subtilis biofilm. Each extract was subjected to a 96-well microplate biofilm inhibition assay, measuring optical density (OD) at 600 nm to quantify biofilm formation. Serial dilution and colony-forming unit (CFU) counting were used to assess bacterial viability. The study found that all three extracts demonstrated notable biofilm inhibition, with the highest inhibition observed at a 10−7dilution for ethanolic extract (89.56%). However, the extracts exhibited inconsistent antibacterial activity, with bacterial colonies too many to count (TMTC) at higher dilutions. Statistical analysis revealed no significant difference in the efficacy among the maceration extraction methods (p = 0.67), confirming that all are equally viable for obtaining bioactive compounds from A. muricata. Regardless of the method used, the extracts effectively inhibited B. subtilis biofilm formation.
https://animorepository.dlsu.edu.ph/conf_shsrescon/2025/paper_fnh/11
